Microarray analysis of selected genes in neural stem and progenitor cells

被引:81
作者
Luo, YQ
Cai, JL
Liu, Y
Xue, HP
Chrest, FJ
Wersto, RP
Rao, M
机构
[1] NIA, Neurosci Lab, Gerontol Res Ctr, Baltimore, MD 21224 USA
[2] NIA, Res Resource Branch, Gerontol Res Ctr, Baltimore, MD 21224 USA
关键词
astrocyte; differentiation; glial restricted precursor; neuroepithelial cells; neuronal restricted precursor; proliferation;
D O I
10.1046/j.1471-4159.2002.01260.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To access and compare gene expression in fetal neuroepithelial cells (NEPs) and progenitor cells, we have used microarrays containing approximately 500 known genes related to cell cycle regulation, apoptosis, growth and differentiation. We have identified 152 genes that are expressed in NEPs and 209 genes expressed by progenitor cells. The majority of genes (141) detected in NEPs are also present in progenitor populations. There are 68 genes specifically expressed in progenitors with little or no expression in NEPs, and a few genes that appear to be present exclusively in NEPs. Using cell sorting, RT-PCR, in situ hybridization or immunocytochemistry, we have examined the segregation of expression to neuronal and glial progenitors, and identified several that appeared to be enriched in neuronal (e.g. CDK5, neuropilin, EphrinB2, FGF11) or glial (e.g. CXCR4, RhoC, CD44, tenascin C) precursors. Our data provide a first report of gene expression profiles of neural stem and progenitor cells at early stages of development, and provide evidence for the potential roles of specific cell cycle regulators, chemokines, cytokines and extracellular matrix molecules in neural development and lineage segregation.
引用
收藏
页码:1481 / 1497
页数:17
相关论文
共 62 条
[1]  
Alfei L, 1999, EUR J HISTOCHEM, V43, P29
[2]  
ALTMAN J, 1984, ADV ANAT EMBRYOL CEL, V85, P1
[3]   The chemokine fractalkine inhibits Fas-mediated cell death of brain microglia [J].
Boehme, SA ;
Lio, FM ;
Maciejewski-Lenoir, D ;
Bacon, KB ;
Conlon, PJ .
JOURNAL OF IMMUNOLOGY, 2000, 165 (01) :397-403
[4]   Properties of a fetal multipotent neural stem cell (NEP cell) [J].
Cai, JL ;
Wu, YY ;
Mirua, T ;
Pierce, JL ;
Lucero, MT ;
Albertine, KH ;
Spangrude, GJ ;
Rao, MS .
DEVELOPMENTAL BIOLOGY, 2002, 251 (02) :221-240
[5]   IMMUNOHISTOCHEMICAL DETECTION OF THROMBOSPONDIN IN MICROGLIA IN THE DEVELOPING RAT-BRAIN [J].
CHAMAK, B ;
DOBBERTIN, A ;
MALLAT, M .
NEUROSCIENCE, 1995, 69 (01) :177-187
[6]   Caspase regulation of neuronal progenitor cell apoptosis [J].
D'Sa-Eipper, C ;
Roth, KA .
DEVELOPMENTAL NEUROSCIENCE, 2000, 22 (1-2) :116-124
[7]   A decade of CDK5 [J].
Dhavan, R ;
Tsai, LH .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2001, 2 (10) :749-759
[8]  
ELKNEFIAWY N, 2002, IMMUNOL CELL BIOL, V80, P441
[9]   ISOLATION, CHARACTERIZATION, AND USE OF STEM-CELLS FROM THE CNS [J].
GAGE, FH ;
RAY, J ;
FISHER, LJ .
ANNUAL REVIEW OF NEUROSCIENCE, 1995, 18 :159-192
[10]  
Garcion E, 2001, DEVELOPMENT, V128, P2485