Molecular cytogenetic analysis of the monoblastic cell line U937: karyotype clarification by G-banding, whole chromosome painting, microdissection and reverse painting, and comparative genomic hybridization

被引：16

作者：

Lee, JY

Lee, CH

Shim, SH

Seo, HK

Kyhm, JH

Cho, S

Cho, YH ^{[1
]}

机构：

[1] Hanyang Univ, Coll Med, Dept Med Genet, Seoul 133791, South Korea

[2] Boston Univ, Sch Med, Ctr Human Genet, Boston, MA 02215 USA

[3] Boston Univ, Sch Med, Dept Pediat, Boston, MA 02215 USA

[4] Univ Kansas, Sch Med, Dept Pediat, Wichita, KS 67214 USA

来源：

CANCER GENETICS AND CYTOGENETICS | 2002年 / 137卷 / 02期

关键词：

D O I：

10.1016/S0165-4608(02)00565-4

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Previous reports on the analysis of the human monoblastic cell line U937 had described several sublines containing unidentified rearrangements and marker chromosomes. In order to determine the true nature of the rearrangements, conventional banding analysis was carried out with various combinations of molecular cytogenetic techniques: comparative genomic hybridization, fluorescence in situ hybridization (FISH) with whole chromosome painting probes, and microdissection and reverse painting FISH. The origins of the marker chromosomes were identified and the composite karyotype is described. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：124 / 132

页数：9

共 51 条

[1] THE CLONED BUTYRYLCHOLINESTERASE (BCHE) GENE MAPS TO A SINGLE CHROMOSOME SITE, 3Q26 [J].