Three-dimensional localization of fluorescent masses deeply embedded in tissue

被引：20

作者：

Eidsath, A

Chernomordik, V

Gandjbakhche, A

Smith, P

Russo, A

机构：

[1] NIH, DBEPS, ORS, Bethesda, MD 20892 USA

[2] NICHD, NIH, Bethesda, MD 20892 USA

[3] NCI, ROB, NIH, Bethesda, MD 20892 USA

来源：

PHYSICS IN MEDICINE AND BIOLOGY | 2002年 / 47卷 / 22期

关键词：

D O I：

10.1088/0031-9155/47/22/311

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this paper we present preliminary results obtained for tissue-like phantoms and ex vivo tissue slabs using a prototype system for CW infrared fluorescence imaging of fluorescent markers. Besides the design of the experimental system itself, we have developed a theoretical model of photon migration under experimental conditions and associated 3D reconstruction algorithms to estimate the distribution of fluorescent markers. Application of the developed algorithms in the analysis of 2D intensity distributions of the fluorescent light demonstrated their ability to reconstruct positions of the markers (including their depths) with good accuracy (error less than or equal to 10%) both for the Intralipid phantoms and ex vivo tissue slabs.

引用

收藏

页码：4079 / 4092

页数：14

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