Efficacy of zinc treatment against iron-induced toxicity in rat hepatoma cell line H4-II-E-C3

被引：18

作者：

Formigari, A. ^{[1
]}

Santon, A. ^{[1
]}

Irato, P. ^{[1
]}

机构：

[1] Univ Padua, Dept Biol, I-35131 Padua, Italy

来源：

LIVER INTERNATIONAL | 2007年 / 27卷 / 01期

关键词：

antioxidant enzymes; cell death; glutathione; metal interactions; metallothionein;

D O I：

10.1111/j.1478-3231.2006.01389.x

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Objective: In this study, we have attempted to explore the possible protection afforded by Zn with regard to its antioxidant potential properties in the iron-induced toxicity. Methods: Rat hepatoma cell line H4-II-E-C3 was treated with 150 mu M ZnSO4, 200 mu M FeSO4 or 150 mu M ZnSO4+ 200 mu M FeSO4 for 24 h. We studied the effect of metallothionein (MT), glutathione and metal (Fe and Zn) accumulation. We evaluated the levels of both MT-1 and MT-2 mRNAs, activities of antioxidant enzymes, and also determined the distribution of MT and cell death index. Results: The cotreatment produced highest levels of MT. Fe concentration was significantly lower in the Zn-Fe-treated cells compared with Fe-treated cells. In both Zn treatments, the superoxide dismutase/glutathione peroxidase ratio was similar to control. The cell death index was lower in the Zn-Fe-treated cells compared with Fe-treated cells. Conclusions: Zn may act as antioxidant by competitive inhibition of iron uptake by the divalent metal transporter and/or by MT induction.

引用

页码：120 / 127

页数：8

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