A new method for determination of buformin in plasma and urine by ion-paired reversed-phase HPLC with ultraviolet detection

被引：6

作者：

Yamamoto, T ^{[1
]}

Kusama, M ^{[1
]}

Matsuno, K ^{[1
]}

Sugiyama, E ^{[1
]}

Yamada, Y ^{[1
]}

Iga, T ^{[1
]}

机构：

[1] Univ Tokyo, Fac Med, Dept Pharm, Univ Tokyo Hosp Bunkyo Ku, Tokyo 1138655, Japan

来源：

BIOMEDICAL CHROMATOGRAPHY | 2002年 / 16卷 / 07期

关键词：

D O I：

10.1002/bmc.182

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Buformin is a widely used as an antidiabetic agent but its renal excretion is still controversial. A new HPLC method with ultraviolet (UV) detection for the determination of buformin in plasma and urine has been developed. After protein precipitation or dilution, buformin and internal standard phenformin were resolved on an octadecyl silica column and detected by UV detection at 233 nm. Intra- and inter-day coefficients of variation were <9%. The limit of quantification was around 0.05 mug/ml for plasma and 2.5 mug/ml for urine. Copyright (C) 2002 John Wiley Sons, Ltd.

引用

页码：453 / 454

页数：2

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