Mutations in Two Independent Pathways Are Sufficient to Create Hermaphroditic Nematodes

被引:63
作者
Baldi, Chris [2 ]
Cho, Soochin [3 ]
Ellis, Ronald E. [1 ]
机构
[1] Univ Med & Dent New Jersey, Sch Osteopath Med, Dept Mol Biol, Stratford, NJ 08084 USA
[2] Univ Med & Dent New Jersey, Grad Sch Biomed Sci, Stratford, NJ 08084 USA
[3] Creighton Univ, Dept Biol, Omaha, NE 68178 USA
基金
美国国家科学基金会;
关键词
CAENORHABDITIS-ELEGANS HERMAPHRODITES; SEX DETERMINATION; REPRODUCTIVE ISOLATION; MOLECULAR ANALYSIS; SPERM ACTIVATION; GERM-LINE; C-ELEGANS; EVOLUTION; GENE; TRA-2;
D O I
10.1126/science.1176013
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Although the nematode Caenorhabditis elegans produces self-fertile hermaphrodites, it descended from a male/female species, so hermaphroditism provides a model for the origin of novel traits. In the related species C. remanei, which has only male and female sexes, lowering the activity of tra-2 by RNA interference created XX animals that made spermatids as well as oocytes, but their spermatids could not activate without the addition of male seminal fluid. However, by lowering the expression of both tra-2 and swm-1, a gene that regulates sperm activation in C. elegans, we produced XX animals with active sperm that were self-fertile. Thus, the evolution of hermaphroditism in Caenorhabditis probably required two steps: a mutation in the sex-determination pathway that caused XX spermatogenesis and a mutation that allowed these spermatids to self-activate.
引用
收藏
页码:1002 / 1005
页数:4
相关论文
共 31 条
[1]  
BAIRD SE, 1992, EVOLUTION, V46, P585, DOI 10.1111/j.1558-5646.1992.tb02067.x
[2]   Reproductive isolation in Caenorhabditis:: terminal phenotypes of hybrid embryos [J].
Baird, SE ;
Yen, WC .
EVOLUTION & DEVELOPMENT, 2000, 2 (01) :9-15
[3]   Detecting heterozygosity in shotgun genome assemblies: Lessons from obligately outcrossing nematodes [J].
Barriere, Antoine ;
Yang, Shiaw-Pyng ;
Pekarek, Elizabeth ;
Thomas, Cristel G. ;
Haag, Eric S. ;
Ruvinsky, Ilya .
GENOME RESEARCH, 2009, 19 (03) :470-480
[4]   The species, sex, and stage specificity of a Caenorhabditis sex pheromone [J].
Chasnov, J. R. ;
So, W. K. ;
Chan, C. M. ;
Chow, K. L. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (16) :6730-6735
[5]  
Chen PJ, 2001, GENETICS, V158, P1513
[6]   A phylogeny of Caenorhabditis reveals frequent loss of introns during nematode evolution [J].
Cho, SC ;
Jin, SW ;
Cohen, A ;
Ellis, RE .
GENOME RESEARCH, 2004, 14 (07) :1207-1220
[7]   SEX DETERMINATION IN THE CAENORHABDITIS ELEGANS GERM LINE [J].
Ellis, Ronald E. .
SEX DETERMINATION AND SEXUAL DEVELOPMENT, 2008, 83 :41-64
[8]   A comparative study of sperm morphology, cytology and activation in Caenorhabditis elegans, Caenorhabditis remanei and Caenorhabditis briggsae [J].
Geldziler, B ;
Chatterjee, I ;
Kadandale, P ;
Putiri, E ;
Patel, R ;
Singson, A .
DEVELOPMENT GENES AND EVOLUTION, 2006, 216 (04) :198-208
[9]   The genetic and molecular analysis of spe-19, a gene required for sperm activation in Caenorhabditis elegans [J].
Geldziler, B ;
Chatterjee, I ;
Singson, A .
DEVELOPMENTAL BIOLOGY, 2005, 283 (02) :424-436
[10]  
GUO Y, CURRENT BIO IN PRESS