Natural infection of Phlebotomus argentipes with Leishmania and other trypanosomatids in a visceral leishmaniasis endemic region of Nepal

被引：39

作者：

Bhattarai, Narayan Raj ^{[1
,2
,3
,4
,5
]}

Das, Murari Lal ^{[4
]}

Rijal, Suman ^{[4
]}

van der Auwera, Gert ^{[1
,2
,3
]}

Picado, Albert ^{[6
]}

Khanal, Basudha ^{[4
]}

Roy, Lalita ^{[4
]}

Speybroeck, Niko ^{[1
,2
,3
,7
]}

Berkvens, Dirk ^{[1
,2
,3
]}

Davies, Clive R. ^{[6
]}

Coosemans, Marc ^{[1
,2
,3
]}

Boelaert, Marleen ^{[1
,2
,3
]}

Dujardin, Jean-Claude ^{[1
,2
,3
,5
]}

机构：

[1] Inst Trop Med, Dept Parasitol, B-2000 Antwerp, Belgium

[2] Inst Trop Med, Dept Publ Hlth, B-2000 Antwerp, Belgium

[3] Inst Trop Med, Dept Vet & Anim Sci, B-2000 Antwerp, Belgium

[4] BP Koirala Inst Hlth Sci, Dharan, Nepal

[5] Univ Antwerp, Dept Biomed Sci, B-2020 Antwerp, Belgium

[6] London Sch Hyg & Trop Med, London WC1, England

[7] Catholic Univ Louvain, Ecole Sante Publ, B-1348 Louvain, Belgium

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 2009年 / 103卷 / 11期

关键词：

Leishmania; Phlebotomus argentipes; PCR; Kala-azar; Ribosomal DNA; Nepal; SANDFLIES DIPTERA; SAND FLIES; PCR; IDENTIFICATION; PSYCHODIDAE; PREVALENCE; DONOVANI; DNA;

D O I：

10.1016/j.trstmh.2009.03.008

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Monitoring Leishmania infection in sand flies is important for understanding the eco-epidemiology of kala-azar and assessing the impact of the recently launched kala-azar control programme in the Indian subcontinent. We applied a PCR technique that targets rRNA genes to estimate the natural incidence of Leishmania infection in sand flies sampled in six villages of the Terai region of Nepal. Amplifications were made on 135 pools of sand flies and confirmed by sequencing. Seven pools were found to be PCR positive: in five of them we identified the rDNA signature found in Leishmania spp., whereas two other pools revealed a sequence compatible with other trypanosomatids. Different methodologies were applied to evaluate the infection rate from pools of unequal size and estimated the infection rate to range from 0.468% to 0.578% for the Leishmania group and from 0.185% to 0.279% for the non-Leishmania group. Our results highlight the diversity of flagellate infections likely to be encountered in Phlebotomus argentipes populations. Our methodology allows clear discrimination of Leishmania from other trypanosomatids and should be applied on larger insect samples or in longitudinal studies. (C) 2009 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.

引用

页码：1087 / 1092

页数：6

共 21 条

[1]

[Anonymous], 1996, J COMPUT GRAPH STAT

[2] Detection and identification of Leishmania DNA within naturally infected sand flies by seminested PCR on minicircle kinetoplastic DNA [J].