Isolation from cattle of a prion strain distinct from that causing bovine spongiform encephalopathy

被引:95
作者
Beringue, Vincent
Bencsik, Anna
Le Dur, Annick
Reine, Fabienne
Lai, Thanh Lan
Chenais, Nathalie
Tilly, Gaelle
Biacabe, Anne-Gaelle
Baron, Thierry
Vilotte, Jean-Luc
Laude, Hubert [1 ]
机构
[1] INRA, Jouy En Josas, France
[2] Agcy Francaise Securite Sanitaire Aliments, Unite Agents Transmissibles Conventionnels, Lyon, France
关键词
D O I
10.1371/journal.ppat.0020112
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
To date, bovine spongiform encephalopathy (BSE) and its human counterpart, variant Creutzfeldt-Jakob disease, have been associated with a single prion strain. This strain is characterised by a unique and remarkably stable biochemical profile of abnormal protease-resistant prion protein (PrPres) isolated from brains of affected animals or humans. However, alternate PrPres signatures in cattle have recently been discovered through large-scale screening. To test whether these also represent separate prion strains, we inoculated French cattle isolates characterised by a PrPres of higher apparent molecular mass-called H-type-into transgenic mice expressing bovine or ovine PrP. All mice developed neurological symptoms and succumbed to these isolates, showing that these represent a novel strain of infectious prions. Importantly, this agent exhibited strain-specific features clearly distinct from that of BSE agent inoculated to the same mice, which were retained on further passage. Moreover, it also differed from all sheep scrapie isolates passaged so far in ovine PrP-expressing mice. Our findings therefore raise the possibility that either various prion strains may exist in cattle, or that the BSE agent has undergone divergent evolution in some animals.
引用
收藏
页码:956 / 963
页数:8
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