An Innovative Triple Immunogold Labeling Method to Investigate the Hemopoietic Stem Cell Niche In Situ

被引：8

作者：

Ellis, Sarah L. ^{[1
,2
,3
]}

Williams, Brenda ^{[1
]}

Asquith, Stephen ^{[2
]}

Bertoncello, Ivan ^{[1
,3
]}

Nilsson, Susan K. ^{[1
,3
]}

机构：

[1] Australian Stem Cell Ctr, Clayton, Vic 3168, Australia

[2] Peter MacCallum Canc Ctr, Microscopy Core Facil, Melbourne, Vic 3002, Australia

[3] Univ Melbourne, Dept Pathol, Parkville, Vic 3052, Australia

来源：

MICROSCOPY AND MICROANALYSIS | 2009年 / 15卷 / 05期

关键词：

hemopoietic stem cell; hemopoietic progenitor cell; homing; niche; immunogold; hyaluronic acid; osteopontin; BONE-MARROW NICHE; ELECTRON-MICROSCOPIC IMMUNOCYTOCHEMISTRY; COLLOIDAL-GOLD; CORRELATIVE MICROSCOPY; PROTEIN-A; MINERALIZED TISSUES; PROGENITOR CELLS; EMBEDDED TISSUE; SILVER METHOD; RAT LUNG;

D O I：

10.1017/S1431927609990924

中图分类号：

T [工业技术];

学科分类号：

120111 [工业工程];

摘要：

The ultrastructural study of rare cells within their niche in situ is very difficult. We have developed a method for locating individual transplanted cells and simultaneously identifying and analyzing the molecules and cellular phenotypes surrounding them in situ using transmission electron microscopy. This innovative method involves triple immunogold labeling combined with serial ultrathin sectioning. We demonstrate the validity of this approach by examining the niche of individual transplanted cells from a population highly enriched for hemopoietic stern cells and the ultrastructural expression of two key stem cell regulatory molecules, hyaluronic acid and osteopontin. In addition, we describe the phenotypes of the surrounding cells.

引用

页码：403 / 414

页数：12

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