Bio-ethanol from water hyacinth biomass: An evaluation of enzymatic saccharification strategy

被引:110
作者
Aswathy, U. S. [1 ]
Sukumaran, Rajeev K. [1 ]
Devi, G. Lalitha [1 ]
Rajasree, K. P. [1 ]
Singhania, Reeta Rani [1 ]
Pandey, Ashok [1 ]
机构
[1] CSIR, Natl Inst Interdisciplinary Sci & Technol, Div Biotechnol, Trivandrum 695019, Kerala, India
关键词
Lignocellulosic biomass; Water hyacinth; Eichhornia; Bio-ethanol; Saccharification; BETA-GLUCOSIDASE; EICHHORNIA-CRASSIPES; HYDROLYSIS; CELLULASE; LIGNOCELLULOSE; BIOCONVERSION; FERMENTATION; ADDITIVES; C30;
D O I
10.1016/j.biortech.2009.08.019
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Biomass feedstock having less competition with food crops are desirable for bio-ethanol production and such resources may not be localized geographically. A distributed production strategy is therefore more suitable for feedstock like water hyacinth with a decentralized availability. In this study, we have demonstrated the suitability of this feedstock for production of fermentable sugars using cellulases produced on site. Testing of acid and alkali pretreatment methods indicated that alkali pretreatment was more efficient in making the sample susceptible to enzyme hydrolysis. Cellulase and beta-glucosidase loading and the effect of surfactants were studied and optimized to improve saccharification. Redesigning of enzyme blends resulted in an improvement of saccharification from 57% to 71%. A crude trial on fermentation of the enzymatic hydrolysate using the common baker's yeast Saccharomyces cerevisiae yielded an ethanol concentration of 4.4 g/L. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:925 / 930
页数:6
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