Estimation of proteins in the presence of polyethylenimine

被引：7

作者：

Gupta, V ^{[1
]}

Nath, S ^{[1
]}

Chand, S ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Biochem Engn & Biotechnol, New Delhi 110016, India

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 11期

关键词：

adsorption; bioseparation; carboxymethyl-cellulose; interference; ion-exchange resin; polyethylenimine; proteins;

D O I：

10.1023/A:1005676531595

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polyethylenimine (PEI) interferes strongly in all commonly employed protein estimation assays. Here, a strategy to remove this interference is developed. Polyethylenimine is selectively removed by adsorption from a mixture containing PEI and lysozyme using carboxymethyl-cellulose, a cation-exchange resin between an ionic strength of 0.35-1 M (pH 6.0), conditions under which lysozyme remains in solution and can be accurately estimated.

引用

页码：927 / 929

页数：3

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