LDL receptor-related protein as a component of the midkine receptor

被引：130

作者：

Muramatsu, H

Zou, K

Sakaguchi, N

Ikematsu, S

Sakuma, S

Muramatsu, T

机构：

[1] Nagoya Univ, Sch Med, Dept Biochem, Showa Ku, Nagoya, Aichi 4668550, Japan

[2] Meiji Milk Prod Co Ltd, Meiji Cell Technol Ctr, Kanagawa 2506862, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 270卷 / 03期

关键词：

brushin; LDL receptor-related protein; midkine; NCAM; neurotrophic factor;

D O I：

10.1006/bbrc.2000.2549

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Midkine (MK) is a heparin-binding growth factor with migration-promoting and survival-promoting activities. To identify signaling receptor(s) of MK, membrane glycoproteins with MK-binding activity were isolated from day 13 mouse embryos by lectin- and MK-affinity chromatography, SDS-PAGE followed by protein sequence analysis revealed the presence of LDL receptor-related protein (LRP) and NCAM in the fraction. The dissociation constant of binding between LRP and MK was 3.5 nM. Receptor-associated protein (RAP), which interfered with the binding, inhibited MK-dependent survival of embryonic neurons. Brushin/megalin, which is also a high molecular weight protein belonging to the LDL receptor family, bound to MK less strongly than LRP. These findings suggest that LRP is a component of the receptor complex for MK. (C) 2000 Academic Press.

引用

页码：936 / 941

页数：6

共 50 条

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