Enhanced RAGE method using specific enrichment by biotinylated oligonucleotides bound to streptavidin coated magnetic particles

被引：6

作者：

Lankiewicz, S ^{[1
]}

Gisselmann, G ^{[1
]}

Hatt, H ^{[1
]}

机构：

[1] RUHR UNIV BOCHUM,LEHRSTUHL ZELLPHYSIOL,D-44780 BOCHUM,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 10期

关键词：

D O I：

10.1093/nar/25.10.2037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RACE (rapid amplification of cDNA ends) is commonly used for identification and isolation of 3' and 5' termini of cDNA. We developed an improvement of the RACE-method that allows the enrichment of wanted fragments. The important new feature is the purification of the amplified products by biotinylated oligonucleotides that hybridize internally. Hybrids are isolated by streptavidin coated magnetic particles.

引用

页码：2037 / 2038

页数：2

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