Molecular and phylogenetic analysis of Cryptosporidium muris from various hosts

被引:51
作者
Morgan, UM [1 ]
Xiao, L
Monis, P
Sulaiman, I
Pavlasek, I
Blagburn, B
Olson, M
Upton, SJ
Khramtsov, NV
Lal, A
Elliot, A
Thompson, RCA
机构
[1] Murdoch Univ, Div Vet & Biomed Sci, State Agr Biotechnol Ctr, Murdoch, WA 6150, Australia
[2] WHO, Collaborating Ctr Mol Epidemiol Parasit Infect, CH-1211 Geneva 27, Switzerland
[3] Ctr Dis Control & Prevent, Div Parasit Dis, Atlanta, GA 30341 USA
[4] Australian Water Qual Ctr, Microbiol Unit, Bolivar, SA 5110, Australia
[5] State Vet Inst Prague, Pathol & Parasitol Dept, Prague 16503 6, Czech Republic
[6] Auburn Univ, Coll Vet Med, Dept Pathobiol, Auburn, AL 36849 USA
[7] Univ Calgary, Calgary, AB, Canada
[8] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA
关键词
Cryptosporidium muris; Cryptosporidium serpentis; 18S rDNA; ITS1; heat-shock gene; phylogenetic analysis;
D O I
10.1017/S0031182099005703
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Isolates of Cryptosporidium muris and C. serpentis were characterized from different hosts using nucleotide sequence analysis of the rDNA 18S and ITS1 regions, and the heat-shock (HSP-70) gene. Phylogenetic analysis confirmed preliminary evidence that C. muris is not a uniform species. Two distinct genotypes were identified within C. muris; (1) C. muris genotype A; comprising bovine and camel isolates of C. muris from different geographical locations, and (2) C. muris genotype B comprising C. muris isolates from mice, a hamster, a rock hyrax and a camel from the same enclosure. These 2 genotypes may represent separate species but further biological and molecular studies are required for confirmation.
引用
收藏
页码:457 / 464
页数:8
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