Induction of RANTES, HLA-DR, and intercellular adhesion molecule-1 on highly purified distal tubular cells from human kidney

被引:21
作者
Baer, PC
Scherberich, JE
Bereiter-Hahn, J
Geiger, H
机构
[1] Univ Frankfurt, Dept Med 4, Div Nephrol, D-60590 Frankfurt, Germany
[2] Univ Frankfurt, Dept Zool, Cinemat Cell Res Grp, D-60590 Frankfurt, Germany
[3] Hosp Munchen Harlaching, Dept Med 2, D-81545 Munich, Germany
关键词
D O I
10.1097/00007890-200006150-00045
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
(B)ackground Expression of proinflammatory molecules by tubular epithelial cells plays an important role in renal allograft rejection and inflammatory kidney diseases. Different studies from patients with acute rejection point to the involvement of distal tubular segments. At present no in vitro system for the human distal tubule is established. Methods. Human distal tubular cells were isolated immunomagnetically. Cultured cells were stimulated with cytokines (interferon-gamma, tumor necrosis factor-alpha, interleukin-1 beta, or a cytokine mix). Secretion of RANTES (regulated upon activation, normal T-cell expressed and secreted) was evaluated with an enzyme-linked immunoassay, Expression of HLA-DR and intercellular adhesion molecule (ICAM)-1 was assessed by flow cytometric analysis and immunofluorescence studies. Results. Our data clearly indicate that distal tubular cells express RANTES, HLA-DR, and ICAM-1 in response to a mixture of specific cytokines. Dexamethasone inhibited the induced expression of RANTES and HLA-DR significantly, but not that of ICAM-1. Conclusions. We demonstrate an appropriate in vitro system for the human distal tubule. The present study proves the involvement of the distal tubular segment during inflammatory kidney diseases.
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收藏
页码:2456 / 2459
页数:4
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