Transforming growth factor-β1 blocks the release of collagen fragments from bovine nasal cartilage stimulated by oncostatin M in combination with IL-1α

被引：17

作者：

Hui, W ^{[1
]}

Rowan, AD ^{[1
]}

Cawston, T ^{[1
]}

机构：

[1] Univ Newcastle Upon Tyne, Sch Med, Dept Rheumatol, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England

来源：

CYTOKINE | 2000年 / 12卷 / 06期

关键词：

cartilage; collagen; collagenases; cytokines; TGF-beta; 1;

D O I：

10.1006/cyto.1999.0625

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oncostatin M in combination with interleukin-l (IL-1) induced a rapid and reproducible release of collagen from bovine nasal cartilage in culture. This release was accompanied by a high collagenolytic activity and low or absent tissue inhibitor of metalloproteinase-1 activity in the culture medium. Transforming growth factor-beta 1 was able to block this release of collagen from the tissue, and reduce the expression and secretion of collagenases whilst maintaining TIMP-1 levels from bovine nasal chondrocytes, This study shows for the first time that TGF-beta 1 can protect cartilage collagen from destruction. (C) 2000 Academic Press.

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收藏

页码：765 / 769

页数：5

相关论文

共 17 条

[1] TRANSFORMING GROWTH FACTOR-BETA CAUSES PARTIAL INHIBITION OF INTERLEUKIN-1 - STIMULATED CARTILAGE DEGRADATION INVITRO [J].

ANDREWS, HJ ;

EDWARDS, TA ;

CAWSTON, TE ;

HAZLEMAN, BL .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 162 (01) :144-150

[2] LUNG TISSUE HYDROLYSATES - STUDIES OF OPTIMUM CONDITIONS FOR SPECTROPHOTOMETRIC DETERMINATION OF HYDROXYPROLINE [J].

BERGMAN, I ;

LOXLEY, R .

ANALYST, 1969, 94 (1120) :575-&

[3] Matrix metalloproteinases and TIMPs: properties and implications for the rheumatic diseases [J].

Cawson, T .

MOLECULAR MEDICINE TODAY, 1998, 4 (03) :130-137

[4] INTERLEUKIN-1 AND ONCOSTATIN-M IN COMBINATION PROMOTE THE RELEASE OF COLLAGEN FRAGMENTS FROM BOVINE NASAL CARTILAGE IN CULTURE [J].

CAWSTON, TE ;

ELLIS, AJ ;

HUMM, G ;

LEAN, E ;

WARD, D ;

CURRY, V .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 215 (01) :377-385

[5]

Cawston TE, 1998, ARTHRITIS RHEUM-US, V41, P1760, DOI 10.1002/1529-0131(199810)41:10<1760::AID-ART8>3.0.CO

[6]

2-M

[7] Induction of matrix metalloproteinase activation cascades based on membrane-type 1 matrix metalloproteinase:: associated activation of gelatinase A, gelatinase B and collagenase 3 [J].

Cowell, S ;

Knäuper, V ;

Stewart, ML ;

d'Ortho, MP ;

Stanton, H ;

Hembry, RM ;

López-Otín, C ;

Reynolds, JJ ;

Murphy, G .

BIOCHEMICAL JOURNAL, 1998, 331 :453-458

[8] TRANSFORMING GROWTH-FACTOR-BETA-1 REGULATES TISSUE INHIBITOR OF METALLOPROTEINASES-1 EXPRESSION IN DIFFERENTIATED HUMAN ARTICULAR CHONDROCYTES [J].

GUNTHER, M ;

HAUBECK, HD ;

VANDELEUR, E ;

BLASER, J ;

BENDER, S ;

GUTGEMANN, I ;

FISCHER, DC ;

TSCHESCHE, H ;

GREILING, H ;

HEINRICH, PC ;

GRAEVE, L .

ARTHRITIS AND RHEUMATISM, 1994, 37 (03) :395-405

[9] Oncostatin M (OSM) stimulates resorption and inhibits synthesis of proteoglycan in porcine articular cartilage explants [J].

Hui, W ;

Bell, M ;

Carroll, G .

CYTOKINE, 1996, 8 (06) :495-500

[10] Detection of oncostatin M in synovial fluid from patients with rheumatoid arthritis [J].

Hui, W ;

Bell, M ;

Carroll, G .

ANNALS OF THE RHEUMATIC DISEASES, 1997, 56 (03) :184-187