Selective activation of cognate SNAREpins by Sec1/Munc18 proteins

被引：363

作者：

Shen, Jingshi ^{[1
]}

Tareste, David C. ^{[1
]}

Paumet, Fabienne ^{[1
]}

Rothman, James E. ^{[1
]}

Melia, Thomas J. ^{[1
]}

机构：

[1] Columbia Univ, Dept Physiol & Cellular Biophys, New York, NY 10032 USA

来源：

CELL | 2007年 / 128卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/j.cell.2006.12.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sec1/Muncl 8 (SM) proteins are required for every step of intracellular membrane fusion, but their molecular mechanism of action has been unclear. In this work, we demonstrate a fundamental role of the SM protein: to act as a stimulatory subunit of its cognate SNARE fusion machinery. In a reconstituted system, mammalian SNARE pairs assemble between bilayers to drive a basal fusion reaction. Munc18-1/nSec1, a synaptic SM protein required for neurotransmitter release, strongly accelerates this reaction through direct contact with both t- and v-SNAREs. Munc18-1 accelerates fusion only for the cognate SNAREs for exocytosis, therefore enhancing fusion specificity.

引用

页码：183 / 195

页数：13

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