Cibotium barometz polysaccharides stimulate chondrocyte proliferation in vitro by promoting G1/S cell cycle transition

被引：41

作者：

Fu, Changlong ^{[1
]}

Zheng, Chunsong ^{[1
,2
]}

Lin, Jie ^{[1
]}

Ye, Jinxia ^{[1
,2
,3
]}

Mei, Yangyang ^{[1
]}

Pan, Caibin ^{[1
]}

Wu, Guangwen ^{[1
,2
]}

Li, Xihai ^{[1
,2
]}

Ye, Hongzhi ^{[1
,2
]}

Liu, Xianxiang ^{[1
,2
]}

机构：

[1] Fujian Univ Tradit Chinese Med, Acad Integrat Med, 1 Qiuyang Rd, Fuzhou 350122, Fujian, Peoples R China

[2] Fujian Univ Tradit Chinese Med, Fujian Prov Key Lab Integrat Med Geriatr, Fuzhou 350122, Fujian, Peoples R China

[3] Fujian Univ Tradit Chinese Med, Acad Integrat Med, Biomed Res Ctr, Fuzhou 350122, Fujian, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2017年 / 15卷 / 05期

关键词：

proliferation; chondrocytes; Cibotium barometz; polysaccharides; DEXTROSE PROLOTHERAPY; OSTEOARTHRITIS; APOPTOSIS;

D O I：

10.3892/mmr.2017.6412

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Cibotium barometz polysaccharides (CBPS) are one of the most important bioactive components extracted from the Cibotium barometz plant, which belongs to the Dicksoniaceae family. It has been widely used for the treatment of orthopedic diseases in traditional Chinese medicine. However, the molecular mechanisms behind the therapeutic effects of CBPS remain to be clarified. In the present study, the concentration of CBPS was detected by phenol-vitriol colorimetry. Furthermore, the effects stimulated by CBPS on the viability and G1/S cell cycle transition in primary chondrocytes from Sprague-Dawley rats were investigated. A cell viability assay demonstrated that chondrocyte proliferation may be enhanced by CBPS in a dose-and time-dependent manner. The mechanism underlying the promotion of chondrocyte cell cycle was suggested to involve the stimulation of G1 to S phase transition. To further confirm the results, reverse transcription-quantitative polymerase chain reaction and western blot analyses were used to detect the expression of mRNA and protein levels of cyclin D1, cyclin-dependent kinase 4 and retinoblastoma protein. The results suggested that CBPS may stimulate chondrocyte proliferation via promoting G1/S cell cycle transition. Since osteoarthritis is characterized by deficient proliferation in chondrocytes, the present study indicates that CBPS may potentially serve as a novel method for the treatment of osteoarthritis.

引用

页码：3027 / 3034

页数：8

共 30 条

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