Direct observation of processive movement by individual myosin V molecules

被引:156
作者
Sakamoto, T
Amitani, I
Yokota, E
Ando, T
机构
[1] Kanazawa Univ, Fac Sci, Dept Phys, Kanazawa, Ishikawa 9201192, Japan
[2] Himeji Inst Technol, Fac Sci, Dept Life Sci, Himeji, Hyogo 6781297, Japan
关键词
motor protein; sliding movement; processivity; ATPase; actin; myosin;
D O I
10.1006/bbrc.2000.2819
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Myosin V is an unconventional myosin thought to move processively along actin filaments. To have hard evidence for the high processivity, we sought to observe directly the movement by individual native chick brain myosin V (BMV) molecules with fluorescent calmodulin, Single BMV molecules did exhibit highly processive movement along actin filaments fixed to a coverslip. BMV continued to move up to the barbed end of its actin track, and did not readily detach from action, The barbed end, therefore, got brighter with time, because of a constant stream of BMV traffic, The maximum speed of the processive movement was 1 mu m/s, and the maximum actin-activated ATPase rate was 2.4 s(-1). These values apparently imply that BMV travels a great distance, 400 nm, per an ATPase cycle. (C) 2000 Academic Press.
引用
收藏
页码:586 / 590
页数:5
相关论文
共 25 条
[1]   Calcium-induced quenching of intrinsic fluorescence in brain myosin V is linked to dissociation of calmodulin light chains [J].
Cameron, LC ;
Carvalho, RN ;
Araujo, JRV ;
Santos, AC ;
Tauhata, SB ;
Larson, RE ;
Sorenson, MM .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1998, 355 (01) :35-42
[2]   The terminal tail region of a yeast myosin-V mediates its attachment to vacuole membranes and sites of polarized growth [J].
Catlett, NL ;
Weisman, LS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) :14799-14804
[3]   Purification and assay of myosin V [J].
Cheney, RE .
MOLECULAR MOTORS AND THE CYTOSKELETON, PT B, 1998, 298 :3-18
[4]   BRAIN MYOSIN-V IS A 2-HEADED UNCONVENTIONAL MYOSIN WITH MOTOR-ACTIVITY [J].
CHENEY, RE ;
OSHEA, MK ;
HEUSER, JE ;
COELHO, MV ;
WOLENSKI, JS ;
ESPREAFICO, EM ;
FORSCHER, P ;
LARSON, RE ;
MOOSEKER, MS .
CELL, 1993, 75 (01) :13-23
[5]   The kinetic mechanism of myosin V [J].
De La Cruz, EM ;
Wells, AL ;
Rosenfeld, SS ;
Ostap, EM ;
Sweeney, HL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (24) :13726-13731
[6]  
ESPEREAFICO EM, 1992, J CELL BIOL, V119, P1541
[7]   BIOCHEMICAL AND IMMUNOLOGICAL CHARACTERIZATION OF P190-CALMODULIN COMPLEX FROM VERTEBRATE BRAIN - A NOVEL CALMODULIN-BINDING MYOSIN [J].
ESPINDOLA, FS ;
ESPREAFICO, EM ;
COELHO, MV ;
MARTINS, AR ;
COSTA, FRC ;
MOOSEKER, MS ;
LARSON, RE .
JOURNAL OF CELL BIOLOGY, 1992, 118 (02) :359-368
[8]  
Evans LL, 1998, J CELL SCI, V111, P2055
[9]  
FISKE CH, 1925, J BIOL CHEM, V14, P1
[10]   Actin and myosin function in directed vacuole movement during cell division in Saccharomyces cerevisiae [J].
Hill, KL ;
Catlett, NL ;
Weisman, LS .
JOURNAL OF CELL BIOLOGY, 1996, 135 (06) :1535-1549