Techniques: Applications of the nerve-bouton preparation in neuropharmacology

被引：141

作者：

Akaike, N

Moorhouse, AJ

机构：

[1] Kyushu Univ, Grad Sch Med Sci, Higashi Ku, Fukuoka 8128582, Japan

[2] Univ New S Wales, Sch Med Sci, Dept Physiol & Pharmacol, Sydney, NSW 2052, Australia

来源：

TRENDS IN PHARMACOLOGICAL SCIENCES | 2003年 / 24卷 / 01期

基金：

日本学术振兴会;

关键词：

D O I：

10.1016/S0165-6147(02)00010-X

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Single mammalian neurons can be isolated with adherent functional synaptic terminals using an enzyme-free, mechanical dissociation procedure. This allows investigations of the effects of presynaptic modulators of synaptic transmission with unprecedented ease and accuracy. Furthermore, single presynaptic terminals and boutons can be visualized using fluorescent markers and can also be focally stimulated with electrical pulses. In this article, the isolated-nerve-adherent-synaptic-bouton preparation and some examples of its general properties and uses are discussed.

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页码：44 / 47

页数：4

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