Zygotic control of maternal cyclin A1 translation and mRNA stability

被引:16
作者
Audic, Y
Garbrecht, M
Fritz, B
Sheets, MD
Hartley, RS
机构
[1] Univ Iowa, Dept Anat & Cell Biol, Iowa City, IA 52242 USA
[2] Univ Wisconsin, Sch Med, Dept Biomol Chem, Madison, WI 53706 USA
关键词
cyclin A1 mRNA; translational control; deadenylation; 3 ' UTR; cell cycle; Xenopus Laevis;
D O I
10.1002/dvdy.10191
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Cyclin mRNAs are unstable in the adult cell cycle yet are stable during the first 12 cell divisions in Xenopus laevis. We recently reported that cyclin A1 and B2 maternal mRNAs are deadenylated upon completion of the 12th division (Audic et al. [2001] Mol. Cell Biol. 21: 1662-1671). Deadenylation is mediated by the 3' untranslated region (UTR) of the mRNA and precedes the terminal disappearance of the cyclin proteins, with both processes requiring zygotic transcription. The purpose of the current study was (1) to ask whether deadenylation. leads to translational repression and/or destabilization of endogenous cyclin A1 and B2 mRNAs, and (2) to further characterize the regulatory sequences required. We show that zygote-driven deadenylation leads to translational repression and mRNA destabilization. A 99-nucleotide region of the 3'UTR of the cyclin A1 mRNA mediates both deadenylation and destabilization. Surprisingly, two AU-rich consensus elements within this region are dispensable for this activity. These results suggest that zygote-dependent deadenylation, translational repression, and mRNA destabilization by means of novel TUTR elements contribute to the disappearance of maternal cyclins. They also suggest that translational control of cyclins may play a role in the transition to the adult cell cycle. These data concur with previous studies in Drosophila showing that zygote-mediated degradation of maternal cdc25 mRNA may be a general mechanism whereby transition to the adult cell cycle proceeds. (C) 2002 Wiley-Liss, Inc.
引用
收藏
页码:511 / 521
页数:11
相关论文
共 62 条
[1]   Control of developmental timing in Caenorhabditis elegans [J].
Ambros, V .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 2000, 10 (04) :428-433
[2]   Postfertilization deadenylation of mRNAs in Xenopus laevis embryos is sufficient to cause their degradation at the blastula stage [J].
Audic, Y ;
Omilli, F ;
Osborne, HB .
MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (01) :209-218
[3]   Zygotic regulation of maternal cyclin A1 and B2 mRNAs [J].
Audic, Y ;
Anderson, C ;
Bhatty, R ;
Hartley, RS .
MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (05) :1662-1671
[4]   Joint action of two RNA degradation pathways controls the timing of maternal transcript elimination at the midblastula transition in Drosophila melanogaster [J].
Bashirullah, A ;
Halsell, SR ;
Cooperstock, RL ;
Kloc, M ;
Karaiskakis, A ;
Fisher, WW ;
Fu, WL ;
Hamilton, JK ;
Etkin, LD ;
Lipshitz, HD .
EMBO JOURNAL, 1999, 18 (09) :2610-2620
[5]   Spatial and temporal control of RNA stability [J].
Bashirullah, A ;
Cooperstock, RL ;
Lipshitz, HD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (13) :7025-7028
[6]  
Brodsky L., 1992, DIMACS SERIES DISCRE, V8, P127
[7]  
BRODSKY LI, 1995, BIOCHEMISTRY-MOSCOW+, V60, P923
[8]  
de Moor CH, 2001, INT REV CYTOL, V203, P567
[9]  
Dickson KS, 1999, MOL CELL BIOL, V19, P5707
[10]   STABILITY OF MATERNAL MESSENGER-RNA IN XENOPUS EMBRYOS - ROLE OF TRANSCRIPTION AND TRANSLATION [J].
DUVAL, C ;
BOUVET, P ;
OMILLI, F ;
ROGHI, C ;
DOREL, C ;
LEGUELLEC, R ;
PARIS, J ;
OSBORNE, HB .
MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (08) :4123-4129