Micellar liquid chromatographic determination of anti-convulsant drugs in pills and capsules

被引:20
作者
Gil-Agustí, M
Carda-Broch, S
García-Alvarez-Coque, MC
Esteve-Romero, J [1 ]
机构
[1] Univ Jaume 1, Area Quim Analit, Castellon de La Plana 12006, Spain
[2] Univ Valencia, Dept Quim Analit, E-46100 Valencia, Spain
关键词
D O I
10.1081/JLC-100100422
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple chromatographic procedure is reported for the determination of several anti-convulsant drugs in pharmaceuticals: carbamazepine, and the benzodiazepines bentazepam, halazepam, oxazepam, pinazepam, and tetrazepam. The procedure utilizes a C-18 column, a hybrid micellar mobile phase of 0.1 M SDS-3% butanol-0.1% triethylamine-0.01 M phosphate buffer (pH 3), and UV detection (230 nm). The drugs eluted in less than 13 min, in accordance to their relative polarities, as indicated by their octanol-water partition coefficients. The limits of detection (mu g/mL), and intra and inter-day repeatabilities (%), for 4 mu g/mL were: carbamazepine (0.03, 1.0, 4.1), bentazepam (0.05, 1.3, 1.6), halazepam (0.10, 2.3, 1.3), oxazepam (0.02, 0.9, 7.9), pinazepam (0.01, 0.3, 5.1), and tetrazepam(0.03, 1.1, 6.3). The results were compared with those obtained in a conventional procedure using methanol-water 70:30 (v/v). The contents evaluated with the micellar mobile phase always agreed with the declared compositions, instead, with the aqueous-organic mobile phase, pinazepam gave poor recoveries and halazepam did not elute at appropriate times.
引用
收藏
页码:1387 / 1401
页数:15
相关论文
共 29 条
[1]   DETERMINATION OF DIAZEPAM AND OXAZEPAM USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND 4TH-DERIVATIVE SPECTROPHOTOMETRIC TECHNIQUES [J].
ABDELHAMID, ME ;
ABUIRJEIE, MA .
ANALYST, 1988, 113 (09) :1443-1446
[2]  
BARGO ES, 1983, J ASSOC OFF ANA CHEM, V66, P864
[3]  
BERTHOD A, IN PRESS MICELLAR LI
[4]   DEVELOPMENT AND PRELIMINARY APPLICATION OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY OF URINARY METABOLITES OF DIAZEPAM IN HUMANS [J].
CHIBA, K ;
HORII, H ;
CHIBA, T ;
KATO, Y ;
HIRANO, T ;
ISHIZAKI, T .
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1995, 668 (01) :77-84
[5]  
CLINELOVE LJ, 1985, J PHARM BIOMED ANAL, V3, P511
[6]  
CYR TD, 1987, J ASSOC OFF ANA CHEM, V70, P836
[7]  
DeLuccia F.J., 1985, LC MAG, V3, P794
[8]   DIRECT SERUM INJECTION WITH MICELLAR LIQUID-CHROMATOGRAPHY FOR THERAPEUTIC DRUG-MONITORING [J].
DELUCCIA, FJ ;
ARUNYANART, M ;
LOVE, LJC .
ANALYTICAL CHEMISTRY, 1985, 57 (08) :1564-1568
[9]   Methods for the measurement of benzodiazepines in biological samples [J].
Drummer, OH .
JOURNAL OF CHROMATOGRAPHY B, 1998, 713 (01) :201-225
[10]   OPTIMIZATION OF THE SEPARATION SELECTIVITY OF A GROUP OF BENZENE AND NAPHTHALENE DERIVATIVES IN MICELLAR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING A C-18 COLUMN AND ALCOHOLS AS MODIFIERS IN THE MOBILE-PHASE [J].
GARCIA, MA ;
VERA, S ;
BOMBIN, M ;
MARINA, ML .
JOURNAL OF CHROMATOGRAPHY, 1993, 646 (02) :297-305