Antigenic characterization of the nucleocapsid protein of Newcastle disease virus by means of a new panel of monoclonal antibodies

被引：10

作者：

Panshin, A

Shihmanter, E

Weisman, Y

Örvell, C

Kydyrmanov, A

Asanov, N

Daulbaeva, K

Sayatov, M

Lipkind, M

机构：

[1] Kimron Vet Inst, Div Avian Dis, IL-50250 Bet Dagan, Israel

[2] Huddinge Univ Hosp, Dept Clin Virol, S-14186 Huddinge, Sweden

[3] Kazakh Acad Sci, Inst Microbiol & Virol, Alma Ata 480021, Kazakhstan

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2000年 / 23卷 / 03期

关键词：

Newcastle disease virus; nucleocapsid protein; antigenic epitopes; monoclonal antibodies; radioimmunoprecipitation assay;

D O I：

10.1016/S0147-9571(99)00077-6

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Nine monoclonal antibodies (MAB) against nucleocapsid protein (NP) of Newcastle disease virus (NDV) have been prepared and characterized. All the MABs were classified into three groups by means of the competitive binding assay. At least three antigenic sites were delineated on the NP, The Ist site includes two closely located epitopes; the 2nd site includes two related and two distinct epitopes; the 3rd site includes two closely related and one distinct epitopes. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：209 / 220

页数：12

共 14 条

[1] Antigenic diversity and similarities detected in avian paramyxovirus type 1 (Newcastle disease virus) isolates using monoclonal antibodies [J].