Gene and enhancer trap transposable elements reveal oxygen deprivation-regulated genes and their complex patterns of expression in Arabidopsis

被引:54
作者
Baxter-Burrell, A
Chang, R
Springer, P
Bailey-Serres, J [1 ]
机构
[1] Univ Calif Riverside, Ctr Plant Cell Biol, Riverside, CA 92521 USA
[2] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA
关键词
transposon tagging; gene expression pattern; anoxia; hypoxia; alcohol dehydrogenase; caffeine; cytosolic calcium; second messenger;
D O I
10.1093/aob/mcf119
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Transposon tagging with modified maize Ds-GUS constructs was used to isolate genes induced by oxygen deprivation in Arabidopsis thaliana. Seedlings of 800 gene-trap (DsG) and 600 enhancer-trap (DsE) lines were grown on vertically positioned plates for 1 week, oxygen deprived for up to 24 h and stained for GUS activity. Oxygen deprivation induced intricate patterns of gene expression in seedlings of 65 lines. The insertion site and phenotypes of 15 lines were examined. Surprisingly, none of the insertions were into genes that encode known anaerobic polypeptides. Insertions were identified within or adjacent to genes encoding proteins of regulatory, enzymatic, mitochondrial protein import and unknown function, as well as adjacent to genes encoding a putative receptor-like kinase and putative sensor-histidine kinase. Four lines had significantly lower ADH activity after 24 h of oxygen deprivation and three of these showed reduced stress tolerance. Two lines with wild-type levels of ADH were low-oxygen intolerant. Paradoxically, several lines had significantly higher ADH activity after 12 h of oxygen deprivation but reduced stress tolerance. Caffeine treatment, which increased ADH specific activity in wild-type seedlings under aerobic conditions, was sufficient to increase GUS staining in seven of the 15 lines, providing evidence that these genes may be regulated by cytosolic calcium levels. These results demonstrate the effectiveness of the Ds-GUS tagging system in the identification of genes that are regulated in response to oxygen deprivation and a calcium second messenger. (C) 2003 Annals of Botany Company.
引用
收藏
页码:129 / 141
页数:13
相关论文
共 30 条
[1]  
ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[2]   Both 5' and 3' sequences of maize adh1 mRNA are required for enhanced translation under low-oxygen conditions [J].
BaileySerres, J ;
Dawe, RK .
PLANT PHYSIOLOGY, 1996, 112 (02) :685-695
[3]   Patterns of protein synthesis and tolerance of anoxia in root tips of maize seedlings acclimated to a low-oxygen environment, and identification of proteins by mass spectrometry [J].
Chang, WWP ;
Huang, L ;
Shen, M ;
Webster, C ;
Burlingame, AL ;
Roberts, JKM .
PLANT PHYSIOLOGY, 2000, 122 (02) :295-317
[4]   Arabidopsis alcohol dehydrogenase expression in both shoots and roots is conditioned by root growth environment [J].
Chung, HJ ;
Ferl, RJ .
PLANT PHYSIOLOGY, 1999, 121 (02) :429-436
[5]   Mutations affecting induction of glycolytic and fermentative genes during germination and environmental stresses in Arabidopsis [J].
Conley, TR ;
Peng, HP ;
Shih, MC .
PLANT PHYSIOLOGY, 1999, 119 (02) :599-607
[6]   Molecular strategies for improving waterlogging tolerance in plants [J].
Dennis, ES ;
Dolferus, R ;
Ellis, M ;
Rahman, M ;
Wu, Y ;
Hoeren, FU ;
Grover, A ;
Ismond, KP ;
Good, AG ;
Peacock, WJ .
JOURNAL OF EXPERIMENTAL BOTANY, 2000, 51 (342) :89-97
[7]   Oxygen deficiency and root metabolism: Injury and acclimation under hypoxia and anoxia [J].
Drew, MC .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1997, 48 :223-250
[8]   Advances in flavonoid research since 1992 [J].
Harborne, JB ;
Williams, CA .
PHYTOCHEMISTRY, 2000, 55 (06) :481-504
[9]   Transduction of an ethylene signal is required for cell death and lysis in the root cortex of maize during aerenchyma formation induced by hypoxia [J].
He, CJ ;
Morgan, PW ;
Drew, MC .
PLANT PHYSIOLOGY, 1996, 112 (02) :463-472
[10]   Biogenesis of Tim proteins of the mitochondrial carrier import pathway: Differential targeting mechanisms and crossing over with the main import pathway [J].
Kurz, M ;
Martin, H ;
Rassow, J ;
Pfanner, N ;
Ryan, MT .
MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (07) :2461-2474