Molecular characterization of imipenem-resistant Pseudomonas aeruginosa in Hiroshima, Japan

被引：26

作者：

Ohara, Masaru

Kouda, Shuntaro

Onodera, Makoto

Fujiue, Yoshihiro

Sasaki, Megurni

Kohara, Tadahiro

Kashiyama, Seiya

Hayashida, Shizue

Kadono, Manami

Komatsuzawa, Hitoshi

Gotoh, Naornasa

Usui, Tuguru

Itaha, Hideyuki

Kuwabara, Masao

Yokoyama, Takashi

Sugai, Motoyuki

机构：

[1] Hiroshima Univ, Grad Sch Biomed Sci, Dept Bacteriol, Hiroshima 7348553, Japan

[2] Hiroshima Univ, Grad Sch Biomed Sci, Dept Urol, Hiroshima 7348553, Japan

[3] Hiroshima Univ Hosp, Dept Clin Lab, Hiroshima 7348551, Japan

[4] Hiroshima Prefectural Hosp, Dept Pathol & Clin Lab Med, Hiroshima 7348530, Japan

[5] Hiroshima City Hosp, Dept Clin Lab, Hiroshima 7308518, Japan

[6] Hiroshima Red Cross Hosp, Dept Clin Lab, Hiroshima 7308619, Japan

[7] Atom Bomb Survivors Hosp, Hiroshima 7308619, Japan

[8] Hiroshima Saiseikai Hosp, Dept Clin Lab, Hiroshima 7314311, Japan

[9] Hiroshima Gen Hosp, Dept Clin Lab, Hiroshima 7380042, Japan

[10] Kyoto Pharmaceut Univ, Dept Microbiol, Kyoto 6078414, Japan

[11] Hiroshima Prefecture Hosp, Dept Internal Med, Hiroshima 7348530, Japan

[12] Aki City Hosp, Hiroshima City Med Assoc, Dept Surg, Hiroshima 7360088, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2007年 / 51卷 / 03期

关键词：

Pseudomonas aeruginosa; drug resistance; metallo-beta-lactamase; molecular epidemiology;

D O I：

10.1111/j.1348-0421.2007.tb03908.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

Pseudomonas aeruginosa showing resistance to imipenem were found in 100 of 1,058 strains (9.5%) from six hospitals (a-f) in Hiroshima City, Japan. Of the 100 strains, 14 (14%) were double disk synergy test positive using sodium mercaptoacetic acid disks, and 18 (18%) were bla(IMP-1) or bla(VIM-2) allele positive by polymerase chain reaction (PCR). Among 100 imipenem-resistant strains, 32 were categorized into multi-drug resistant strains, in which 13 were positive for the metallo-p-lactamase gene. Fifty-one strains (51%) among the 100 imipenem-resistant strains had elevated RND efflux pump activity against levofloxacin. But only 6 of 51 strains were classified as multi-drug resistant strains. The pulsed field gel electrophoresis analysis of the SpeI-digested DNA from the 100 isolates suggested not only clonal spread but spread of heterogeneous clones started to contribute to the prevalence of metallo-beta-lactamase producing beta aeruginosa strains in Japanese hospitals.

引用

页码：271 / 277

页数：7

共 28 条

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[2]

Molecular characterization of a β-lactamase gene, blaGIM-1, encoding a new subclass of metallo-β-lactamase [J].