A Validated HPLC Method for Estimation of Cordifolioside A in Tinospora cordifolia, Miers and Marketed Formulations

A simple, economic, robust, reproducible, selective, and precise high-performance liquid chromatography (HPLC) method for estimation of cordifolioside A in both 60% methanolic extract of Tinospora cordifolia and marketed formulation is developed and validated in the present study. The mobile phase composed of acetonitrile-water (25:75%, v/v) give a sharp and well-defined peak of cordifolioside A at the retention time of 9.52 ± 1.03 min. HPLC estimation of cordifolioside A is carried out at wavelength of 210 nm with flow rate of 1.0 mL/min. The linear regression analysis data for the calibration curve shows good linear relationship with correlation coefficient of 0.997 in the concentration range of 0.5-50 μg/mL. The linear regression equation is y = 29716x - 4417.4. The limit of detection and limit of quantification are 0.18 and 0.55 μg/mL, respectively. The developed method is validated for accuracy, precision, reproducibility, and robustness as per ICH guidelines. The proposed method with high degree of precision and accuracy is employed for the estimation of cordifolioside A in methanolic extract and in formulation. Statistical analysis proved that the method is precise, reproducible, selective, and accurate for the estimation of cordifolioside A.