Phospholipase D activation by endogenous 5-hydroxytryptamine 2C receptors is mediated by Gα13 and pertussis toxin-insensitive Gβγ subunits

被引：54

作者：

McGrew, L ^{[1
]}

Chang, MSS ^{[1
]}

Sanders-Bush, E ^{[1
]}

机构：

[1] Vanderbilt Univ, Sch Med, Dept Pharmacol, Nashville, TN 37232 USA

来源：

MOLECULAR PHARMACOLOGY | 2002年 / 62卷 / 06期

关键词：

D O I：

10.1124/mol.62.6.1339

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Phospholipase D activation was measured in primary cultures of rat choroid plexus epithelial cells, which endogenously express the 5-hydroxytryptamine (5-HT) 2C receptor, as well as a heterologous cell line expressing the cloned receptor. In both systems, serotonin stimulation of the 5-HT2C receptor activates phospholipase D in addition to phospholipase C, the traditional effector. Specific inhibitors and membrane permeable blocking peptides were used to determine which heterotrimeric G-proteins were involved. Results suggest that both alpha and free betagamma subunits from G(13) heterotrimers are responsible for phospholipase D activation.

引用

页码：1339 / 1343

页数：5

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