Development of Vero cell-derived inactivated Japanese encephalitis vaccine

被引：71

作者：

Sugawara, K ^{[1
]}

Nishiyama, K ^{[1
]}

Ishikawa, Y ^{[1
]}

Abe, M ^{[1
]}

Sonoda, K ^{[1
]}

Komatsu, K ^{[1
]}

Horikawa, Y ^{[1
]}

Takeda, K ^{[1
]}

Honda, T ^{[1
]}

Kuzuhara, S ^{[1
]}

Kino, Y ^{[1
]}

Mizokami, H ^{[1
]}

Mizuno, K ^{[1
]}

Oka, T ^{[1
]}

Honda, K ^{[1
]}

机构：

[1] Kikuchi Res Ctr, Chemosero Therapeut Res Inst, Kumamoto 8691298, Japan

来源：

BIOLOGICALS | 2002年 / 30卷 / 04期

关键词：

Japanese encephalitis vaccine; Vero cell; microcarrier;

D O I：

10.1006/biol.2002.0345

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have established a manufacturing system for a Vero cell-derived inactivated Japanese encephalitis vaccine at a 500 \ scale. The production system involves expansion of Vero cells using microcarrier, followed by virus infection. Except for an additional purification step, the downstream purification processes are similar to those used for the current mouse brain-derived vaccine; cell removal, concentration and removal of low-molecular weight impurities by membrane filtration, formal in-inactivation, sucrose density gradient ultracentrifugation, and Sulfate-Cellulofine column chromatography are conducted. The antigen obtained from the manufacturing system was highly purified and its physico-chemical and immunological properties were comparable with those of antigen derived from mouse brains. Our system is very simple and could be easily scaled-up to allow vaccine production at a several thousand litre scale. (C) 2002 The International Association for Biologicals. Published by Elsevier Science Ltd. All rights reserved.

引用

页码：303 / 314

页数：12

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