Stiffness of Hydrogels Regulates Cellular Reprogramming Efficiency Through Mesenchymal-to-Epithelial Transition and Stemness Markers

被引：61

作者：

Choi, Bogyu ^{[1
]}

Park, Kwang-Sook ^{[1
,2
]}

Kim, Ji-Ho ^{[1
]}

Ko, Kyoung-Won ^{[1
]}

Kim, Jin-Su ^{[1
]}

Han, Dong Keun ^{[2
]}

Lee, Soo-Hong ^{[1
]}

机构：

[1] CHA Univ, Dept Biomed Sci, 335 Pangyo Ro, Songnam 463400, Gyeonggi Do, South Korea

[2] Korea Inst Sci & Technol, Ctr Biomat, POB 131, Seoul 130650, South Korea

来源：

MACROMOLECULAR BIOSCIENCE | 2016年 / 16卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

cellular reprogramming; induced pluripotent stem cells; mesenchymal-to-epithelial transition; stemness markers; stiffness of hydrogel; E-CADHERIN; SUBSTRATE STIFFNESS; MATRIX STIFFNESS; N-CADHERIN; CELLS; MOUSE; EMT; APOPTOSIS; SWITCH; OCT4;

D O I：

10.1002/mabi.201500273

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The stiffness of hydrogels has been reported to direct cell fate. Here, we found that the stiffness of hydrogels promotes the reprogramming of mouse embryonic fibroblasts into induced pluripotent stem cells (iPSCs). We prepared cell culture substrates of various stiffnesses (0.1, 1, 4, 10, and 20kPa) using a polyacrylamide hydrogel. We found that culture on a soft hydrogel plays an important role in inducing cellular reprogramming into iPSCs via activation of mesenchymal-to-epithelial transition and enhancement of stemness marker expression. These results suggest that physical signals at the interface between cell and substrate can be used as a potent regulator to promote cell fate changes associated with reprogramming into iPSCs, which may lead to effective and reproducible iPSC-production.

引用

页码：199 / 206

页数：8

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