The distribution of receptors for the pro-inflammatory cytokines interleukin (IL)-6 and IL-8 in the developing human fetus

被引:54
作者
Dame, JB [1 ]
Juul, SE [1 ]
机构
[1] Univ Florida, Coll Med, Dept Pediat, Gainesville, FL 32610 USA
关键词
pro-inflammatory cytokines; interleukin-6; interleukin-8; human fetus; cytokine receptors; tissue distribution; cellular expression;
D O I
10.1016/S0378-3782(00)00064-5
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Interleukin-6 receptor oc (IL-6R) and interleukin-8 receptor (IL-8RB) are widely expressed in adult human and murine tissues. Little is known about the expression of these receptors and the function of their ligands in the developing human fetus. Objectives: To determine the tissue distribution and cellular expression of IL-6R and IL-8RB in the developing human fetus. Methods: Reverse transcription-PCR and immunohistochemical staining were performed on brain, spinal cord, eye, heart, lung, liver, spleen, adrenal, kidney, intestine, and placenta from fetuses of 8 and 16+/-2 weeks post-conception. Results: IL-6R and IL-8RB mRNA were detected in all tissues tested at both time points. Immunoreactivity to anti-IL-6R antibody was present on neurons, and in neuropil of the brain, as well as in bone marrow, bronchi, hepatocytes, zona glomerulosa of the adrenal, glomerular cells in kidney, spleen, and placental trophoblasts. Cell-specific expression for IL-8RB in the central nervous system was localized to specific groups of neurons and astrocytes in the brain and spinal cord, including the neural retina. In somatic organs IL-8RB was detected in bone marrow, myocardiocytes, bronchiolar epithelial cells, hepatocytes, cells of the zona glomerulosa and the zona fasciculata of the adrenal, the collecting system of the kidney, enterocytes of the bowel and in placental cells. Conclusion: The widespread expression of these cytokine receptors suggests nonhematopoietic role for their ligand in the developing fetus. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:25 / 39
页数:15
相关论文
共 68 条
  • [1] CYTOKINE REGULATION OF ASTROCYTE FUNCTION - IN-VITRO STUDIES USING CELLS FROM THE HUMAN BRAIN
    ALOISI, F
    BORSELLINO, G
    CARE, A
    TESTA, U
    GALLO, P
    RUSSO, G
    PESCHLE, C
    LEVI, G
    [J]. INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, 1995, 13 (3-4) : 265 - 274
  • [2] IL-6 deficiency leads to increased emotionality in mice:: evidence in transgenic mice carrying a null mutation for IL-6
    Armario, A
    Hernández, J
    Bluethmann, H
    Hidalgo, J
    [J]. JOURNAL OF NEUROIMMUNOLOGY, 1998, 92 (1-2) : 160 - 169
  • [3] Atta-ur-Rahman, 1999, CURR PHARM DESIGN, V5, P241
  • [4] REGULATION OF INTERLEUKIN-6, OSTEOCLASTOGENESIS, AND BONE MASS BY ANDROGENS THE ROLE OF THE ANDROGEN RECEPTOR
    BELLIDO, T
    JILKA, RL
    BOYCE, BF
    GIRASOLE, G
    BROXMEYER, H
    DALRYMPLE, SA
    MURRAY, R
    MANOLAGAS, SC
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1995, 95 (06) : 2886 - 2895
  • [5] Distribution of granulocyte colony-stimulating factor (G-CSF) and G-CSF-receptor mRNA and protein in the human fetus
    Calhoun, DA
    Donnelly, WH
    Du, Y
    Dame, JB
    Li, Y
    Christensen, RD
    [J]. PEDIATRIC RESEARCH, 1999, 46 (03) : 333 - 338
  • [6] Transforming growth factor beta 1, interleukin-8 and interleukin-1 in non-small-cell lung tumors
    Colasante, A
    Mascetra, N
    Brunetti, M
    Lattanzio, G
    Diodoro, M
    Caltagirone, S
    Musiani, P
    Aiello, FB
    [J]. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1997, 156 (03) : 968 - 973
  • [7] Expression of the interleukin 6 receptor in primary renal cell carcinoma
    Costes, V
    Liautard, J
    Picot, MC
    Robert, M
    Lequeux, N
    Brochier, J
    Baldet, P
    Rossi, JF
    [J]. JOURNAL OF CLINICAL PATHOLOGY, 1997, 50 (10) : 835 - 840
  • [8] The distribution of granulocyte-macrophage colony-stimulating factor and its receptor in the developing human fetus
    Dame, JB
    Christensen, RD
    Juul, SE
    [J]. PEDIATRIC RESEARCH, 1999, 46 (04) : 358 - 366
  • [9] Interleukin-8 receptor modulates IgE production and B-cell expansion and trafficking in allergen-induced pulmonary inflammation
    de Sanctis, GT
    MacLean, JA
    Qin, SX
    Wolyniec, WW
    Grasemann, H
    Yandava, CN
    Jiao, AP
    Noonan, T
    Stein-Streilein, J
    Green, FHY
    Drazen, JM
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1999, 103 (04) : 507 - 515
  • [10] Upregulation of interleukin 8 by oxygen-deprived cells in glioblastoma suggests a role in leukocyte activation, chemotaxis, and angiogenesis
    Desbaillets, I
    Diserens, AC
    deTribolet, N
    Hamou, MF
    Van Meir, EG
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1997, 186 (08) : 1201 - 1212