Isolation of Escherichia coli O157:H7 from dung beetles Catharsius molossus

被引：20

作者：

Xu, JG ^{[1
]}

Liu, QY

Jing, HQ

Pang, B

Yang, JC

Zhao, GF

Li, HW

机构：

[1] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Prevent & Control, Beijing 102206, Peoples R China

[2] Ctr Dis Control, Xuzhou, Jiangsu Provinc, Peoples R China

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2003年 / 47卷 / 01期

关键词：

Escherichia coli O157 : H7; beetles; animal;

D O I：

10.1111/j.1348-0421.2003.tb02784.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In an epidemiological survey, Escherichia coli O157:117 was isolated from the intestine 4 of 113 dung beetle Catharsius molossus captured below ground at Tongshan County, Jiangsu Province of China. In parallel, 10 strains of E. coli O157:117 were isolated from fecal samples of 383 diarrhea patients from the same region. Most importantly, using pulsed field gel electrophoresis (PFGE) of chromosomal DNA restriction fragments and PCR method, we found that the PFGE pattern and virulence genes of beetle isolates were identical to those of the human isolates, such as Shiga-toxins (stx) and enterohemorrhagic Escherichia coli hemolysin A (EHEC-hlyA). Therefore, dung beetle might acquire pathogenic E. coli O157:117 through contact with feces of domestic animals.

引用

页码：45 / 49

页数：5

共 29 条

[1] Emerging foodborne diseases [J].

Altekruse, SF ;

Cohen, ML ;

Swerdlow, DL .

EMERGING INFECTIOUS DISEASES, 1997, 3 (03) :285-293

[2]

Baljer G, 1999, DEUT TIERARZTL WOCH, V106, P339

[3]

Barker J, 1999, FEMS MICROBIOL LETT, V173, P291, DOI 10.1111/j.1574-6968.1999.tb13516.x

[4] The isolation and detection of Escherichia coli O157 by use of immunomagnetic separation and immunoassay procedures [J].

Bennett, AR ;

MacPhee, S ;

Betts, RP .

LETTERS IN APPLIED MICROBIOLOGY, 1996, 22 (03) :237-243

[5]

BORCZYK AA, 1987, LANCET, V1, P98

[6] An outbreak of infection due to verocytotoxin-producing Escherichia coli O157 in four families: the influence of laboratory methods on the outcome of the investigation [J].

Chapman, PA ;

Siddons, CA ;

Manning, J ;

Cheetham, C .

EPIDEMIOLOGY AND INFECTION, 1997, 119 (02) :113-119

[7]

Chapman PA, 1996, VET REC, V139, P427

[8] Are all infections with Escherichia coli O157 associated with cattle? [J].

Chart, H .

LANCET, 1998, 352 (9133) :1005-1005

[9] Leaching of Escherichia coli O157:H7 in diverse soils under various agricultural management practices [J].

Gagliardi, JV ;

Karns, JS .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (03) :877-883

[10] Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains [J].

Gannon, VPJ ;

DSouza, S ;

Graham, T ;

King, RK ;

Rahn, K ;

Read, S .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (03) :656-662

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