Sensitive ESR determination of intracellular oxidative stress by using acyl-protected hydroxylamines as new spin reagents

被引:15
作者
Itoh, O [1 ]
Aoyama, M [1 ]
Yokoyama, H [1 ]
Obara, H [1 ]
Ohya, H [1 ]
Kamada, H [1 ]
机构
[1] Yamagata Techniopolis Fdn, Inst Life Support Technol, Yamagata 9902473, Japan
关键词
D O I
10.1246/cl.2000.304
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Several acyl-protected hydroxylamines were synthesized as new spin reagents for ESR measurements of intracellular oxidative stress. These compounds were stable non-radical compounds, but were easily deprotected with esterase to yield hydroxylamines, which were oxidized by oxidants to yield ESR- detectable nitroxide radical. Using an acyl-protected hydroxylamine, a highly sensitive ESR determination procedure was successfully conducted to analyze oxidative stress in human leukocytes.
引用
收藏
页码:304 / 305
页数:2
相关论文
共 14 条
[1]  
BRITIGAN BE, 1986, J BIOL CHEM, V261, P4426
[2]   SPIN TRAPPING - ELECTRON-SPIN-RESONANCE PARAMETERS OF SPIN ADDUCTS [J].
BUETTNER, GR .
FREE RADICAL BIOLOGY AND MEDICINE, 1987, 3 (04) :259-303
[3]   OXIDATION OF HYDROXYLAMINES TO NITROXIDE SPIN LABELS IN LIVING CELLS [J].
CHEN, K ;
SWARTZ, HM .
BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 970 (03) :270-277
[4]   Quantification of peroxynitrite, superoxide, and peroxyl radicals by a new spin trap hydroxylamine 1-hydroxy-2,2,6,6-tetramethyl-4-oxo-piperidine [J].
Dikalov, S ;
Skatchkov, M ;
Bassenge, E .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 230 (01) :54-57
[5]   PRODUCTION OF HYDROXYL AND SUPEROXIDE RADICALS BY STIMULATED HUMAN-NEUTROPHILS - MEASUREMENTS BY EPR SPECTROSCOPY [J].
GREEN, MR ;
HILL, HAO ;
OKOLOWZUBKOWSKA, MJ ;
SEGAL, AW .
FEBS LETTERS, 1979, 100 (01) :23-26
[6]  
KALAI T, 1995, SYNTHESIS-STUTTGART, P1278
[7]   NEWER ASPECTS OF SYNTHESIS AND CHEMISTRY OF NITROXIDE SPIN LABELS [J].
KEANA, JFW .
CHEMICAL REVIEWS, 1978, 78 (01) :37-64
[8]  
MAKINO R, 1986, J BIOL CHEM, V261, P1444
[9]  
Sato EF, 1996, METHOD ENZYMOL, V268, P503
[10]   SATURATED AND TRANS-UNSATURATED FATTY-ACIDS ELICIT HIGH-LEVELS OF SUPEROXIDE GENERATION IN INTACT AND CELL-FREE PREPARATIONS OF NEUTROPHILS [J].
TANAKA, T ;
KANEGASAKI, S ;
MAKINO, R ;
IIZUKA, T ;
ISHIMURA, Y .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 144 (02) :606-612