Whole-Cell Impedance Analysis for Highly and Poorly Metastatic Cancer Cells

被引：62

作者：

Cho, Younghak ^{[1
]}

Kim, Hyun Soo ^{[2
]}

Frazier, A. Bruno ^{[3
]}

Chen, Zhuo G. ^{[4
]}

Shin, Dong Moon ^{[4
]}

Han, Arum ^{[2
]}

机构：

[1] Seoul Natl Univ Technol, Sch Mech Design & Automat Engn, Seoul 139743, South Korea

[2] Texas A&M Univ, Dept Elect & Comp Engn, College Stn, TX 77843 USA

[3] Georgia Inst Technol, Sch Elect & Comp Engn, Atlanta, GA 30332 USA

[4] Emory Univ, Winship Canc Inst, Atlanta, GA 30332 USA

来源：

JOURNAL OF MICROELECTROMECHANICAL SYSTEMS | 2009年 / 18卷 / 04期

关键词：

Cancer detection; electrical impedance spectroscopy (EIS); head and neck cancer (HNC) cell; STEM-CELLS; DIELECTRIC-SPECTROSCOPY; MICROFLUIDIC DEVICE; BIOLOGICAL CELLS; FLOW CYTOMETER; MICROARRAY; MARKERS; MODEL; HEAD;

D O I：

10.1109/JMEMS.2009.2021821

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A micro electrical impedance spectroscopy (mu EIS) system has been developed and implemented to analyze highly and poorly metastatic head and neck cancer (HNC) cell lines with single-cell resolution. The microsystem has arrays of 16 impedance analysis sites, each of which is capable of capturing a single cell and analyzing its whole-cell electrical impedance spectrum. This mu EIS system was used to obtain the electrical impedance spectra of the poorly metastatic HNC cell line 686LN and the highly metastatic HNC cell line 686LN-M4e over a frequency range of 40 Hz-10 MHz. The 686LN cells had higher impedance phase compared to that of 686LN-M4e cells at frequencies between 50 kHz and 2 MHz. This result demonstrates that the metastatic state of HNC cells can be distinguished using the developed mu EIS system. This system is expected to serve as a powerful tool for future detection and quantification of cancer cells from various tumor stages.

引用

页码：808 / 817

页数：10

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