The conformational preference of gramicidin channels is a function of lipid bilayer thickness

被引：111

作者：

Mobashery, N ^{[1
]}

Nielsen, C ^{[1
]}

Andersen, OS ^{[1
]}

机构：

[1] CORNELL UNIV, COLL MED, DEPT PHYSIOL & BIOPHYS, NEW YORK, NY 10021 USA

来源：

FEBS LETTERS | 1997年 / 412卷 / 01期

关键词：

hydrophobic matching; membrane deformation energy; single stranded channel; double stranded channel;

D O I：

10.1016/S0014-5793(97)00709-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to understand how the material properties of lipid bilayers could affect integral membrane protein function, we examined the effect of a hydrophobic mismatch on the structure and function of membrane-spanning gramicidin channels. Changes in lipid bilayer thickness affect the conformational preference of membrane-spanning gramicidin A (gA) channels (single-stranded [SS] dimers <-> double-stranded [DS] diners) and induces an additional conductance state in the standard (SS) beta(6.3)-helical channel. These results provide experimental evidence for the importance of energetic coupling between the bilayer and imbedded inclusions. (C) 1997 Federation of European Biochemical Societies.

引用

页码：15 / 20

页数：6

共 34 条

[1]

Andersen O.S, 1992, BIOMEMBRANE STRUCTUR, P227

[2] MOLECULAR DETERMINANTS OF CHANNEL FUNCTION [J].

ANDERSEN, OS ;

KOEPPE, RE .

PHYSIOLOGICAL REVIEWS, 1992, 72 (04) :S89-S158

[3] ION MOVEMENT THROUGH GRAMICIDIN-A CHANNELS - SINGLE-CHANNEL MEASUREMENTS AT VERY HIGH POTENTIALS [J].

ANDERSEN, OS .

BIOPHYSICAL JOURNAL, 1983, 41 (02) :119-133

[4] H-1-NMR STUDY OF GRAMICIDIN-A TRANSMEMBRANE ION CHANNEL - HEAD-TO-HEAD RIGHT-HANDED, SINGLE-STRANDED HELICES [J].

ARSENIEV, AS ;

BARSUKOV, IL ;

BYSTROV, VF ;

LOMIZE, AL ;

OVCHINNIKOV, YA .

FEBS LETTERS, 1985, 186 (02) :168-174

[5] MODULATION OF PROTEIN FUNCTION BY LIPIDS [J].

BIENVENUE, A ;

STMARIE, J .

CELL LIPIDS, 1994, 40 :319-354

[6] MODULATION OF RHODOPSIN FUNCTION BY PROPERTIES OF THE MEMBRANE BILAYER [J].

BROWN, MF .

CHEMISTRY AND PHYSICS OF LIPIDS, 1994, 73 (1-2) :159-180

[7] ON THE CONDUCTANCE HETEROGENEITY IN MEMBRANE CHANNELS FORMED BY GRAMICIDIN-A - A COOPERATIVE STUDY [J].

BUSATH, DD ;

ANDERSEN, OS ;

KOEPPE, RE .

BIOPHYSICAL JOURNAL, 1987, 51 (01) :79-88

[8] H-2-NUCLEAR MAGNETIC-RESONANCE INVESTIGATIONS ON PHOSPHOLIPID ACYL CHAIN ORDER AND DYNAMICS IN THE GRAMICIDIN-INDUCED HEXAGONAL HII PHASE [J].

CHUPIN, V ;

KILLIAN, JA ;

DEKRUIJFF, B .

BIOPHYSICAL JOURNAL, 1987, 51 (03) :395-405

[9] SPECIFICITY OF LIPID-PROTEIN INTERACTIONS AS DETERMINED BY SPECTROSCOPIC TECHNIQUES [J].

DEVAUX, PF ;

SEIGNEURET, M .

BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 822 (01) :63-125

[10] FORMATION OF NON-BETA-6.3-HELICAL GRAMICIDIN CHANNELS BETWEEN SEQUENCE-SUBSTITUTED GRAMICIDIN ANALOGS [J].

DURKIN, JT ;

PROVIDENCE, LL ;

KOEPPE, RE ;

ANDERSEN, OS .

BIOPHYSICAL JOURNAL, 1992, 62 (01) :145-159

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