Cloudy apple juice is more effective than apple polyphenols and an apple juice derived cloud fraction in a rat model of colon carcinogenesis

被引:44
作者
Barth, Stephan W.
Faehndrich, Christine
Bub, Achim
Watzl, Bernhard
Will, Frank
Dietrich, Helmut
Rechkemmer, Gerhard
Briviba, Karlis
机构
[1] Fed Res Ctr Nutr & Food, Inst Nutr Physiol, D-76131 Karlsruhe, Germany
[2] Tech Univ Munich, Dept Food & Nutr, D-85354 Freising Weihenstephan, Germany
[3] Geisenheim Res Ctr, Dept Wine Anal & Beverage Res, D-65366 Geisenheim, Germany
关键词
dimethylhydrazine; genotoxicity; proliferation; aberrant crypt foci; rat; cloudy apple juice;
D O I
10.1021/jf063078t
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
As recently shown, a cloudy apple juice (CloA) was effective to modulate colon cancer associated parameters in rats treated with 1,2-dimethylhydrazine (DMH). To identify the bioactive substance classes in CloA, we fractionated CloA to yield a total polyphenol (PF) and a cloud (CF) fraction consisting of proteins, fatty acids, polyphenols, and cell wall polysaccharides. Rats received water (control (Cont)) or CloA, PF, and CF separate or combined (PF-CF) ad libitum for 7 weeks starting one week before the first DMH-injection. As determined by comet assay, the DMH-induced genotoxicity in colonocytes of controls (Cont/DMH: 7.7 +/- 0.5%) was significantly reduced by CloA (3.3 +/- 0.3%) but not by any of the fractions. The crypt cell proliferation induced by DMH (Cont/NaCl: 7.5 +/- 0.6%; Cont/DMH: 14.9 +/- 0.8%) was significantly decreased by CloA (9.4 +/- 0.4%), PF (12.4 +/- 0.7%), CF (11.6 +/- 0.4%), and PF-CF (12.4 +/- 0.6%). Although not statistically significant, CloA tended to reduce the number of large aberrant crypt foci (ACF) (Cont/DMH: 19.0 +/- 3.7; CloA/DMH: 12.3 +/- 1.9), while none of the fractions affected ACFs. Neither CloA nor the fractions changed mRNAs of colonic cyclooxygenases (COX-1, COX-2), glutathione-associated enzymes (GST-M2, gamma-GCS, GST-P), the splenocyte CD4/CD8 ratio, natural killer cell activity, and plasma antioxidant status. These results demonstrate that CloA had a higher cancer-preventive potential than the fractions and further, besides PF, identified CF as an additional bioactive fraction of CloA.
引用
收藏
页码:1181 / 1187
页数:7
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