In vivo analysis of metal distribution and expression of metal transporters in rice seed during germination process by microarray and X-ray Fluorescence Imaging of Fe, Zn, Mn, and Cu

被引：91

作者：

Takahashi, Michiko ^{[3
]}

Nozoye, Tomoko ^{[3
]}

Kitajima, Nobuyuki ^{[4
]}

Fukuda, Naoki ^{[5
]}

Hokura, Akiko ^{[5
]}

Terada, Yasuko ^{[2
]}

Nakai, Izumi ^{[5
]}

Ishimaru, Yasuhiro ^{[3
]}

Kobayashi, Takanori ^{[3
]}

Nakanishi, Hiromi ^{[3
]}

Nishizawa, Naoko K. ^{[1
,3
]}

机构：

[1] Univ Tokyo, Dept Global Agr Sci, Plant Biotechnol Lab, Bunkyo Ku, Tokyo 1138657, Japan

[2] JASRI, Sayo, Hyogo 6795198, Japan

[3] Univ Tokyo, Grad Sch Agr & life Sci, Bunkyo Ku, Tokyo 1138657, Japan

[4] Fujita Corp, Atsugi, Kanagawa 2430125, Japan

[5] Tokyo Univ Sci, Dept Appl Chem, Shinjuku Ku, Tokyo 1628601, Japan

来源：

PLANT AND SOIL | 2009年 / 325卷 / 1-2期

关键词：

Germination; Rice seed; Transporter; Synchrotron-based X-ray microfluorescence; Metal nutrients; ARABIDOPSIS-THALIANA; IRON UPTAKE; COPPER TRANSPORTER; GENE-EXPRESSION; NICOTIANAMINE; ZINC; PROTEIN; HOMEOSTASIS; BARLEY; PLANT;

D O I：

10.1007/s11104-009-0045-7

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

To investigate the flow of the metal nutrients iron (Fe), zinc (Zn), manganese (Mn), and copper (Cu) during rice seed germination, we performed microarray analysis to examine the expression of genes involved in metal transport. Many kinds of metal transporter genes were strongly expressed and their expression levels changed during rice seed germination. We found that metal transporter genes such as ZIP family has tendency to decrease in their expressions during seed germination. Furthermore, imaging of the distribution of elements (Fe, Mn, Zn, and Cu) was carried out using Synchrotron-based X-ray microfluorescence at the Super Photon ring-8 GeV (SPring-8) facility. The change in the distribution of each element in the seeds following germination was observed by in vivo monitoring. Iron, Mn, Zn, and Cu accumulated in the endosperm and embryos of rice seeds, and their distribution changed during rice seed germination. The change in the patterns of mineral localization during germination was different among the elements observed.

引用

页码：39 / 51

页数：13

共 55 条

[1] Inventory of the superfamily of P-type ion pumps in Arabidopsis [J].

Axelsen, KB ;

Palmgren, MG .

PLANT PHYSIOLOGY, 2001, 126 (02) :696-706

[2] EFFECT OF ZINC NUTRITIONAL-STATUS ON GROWTH, PROTEIN-METABOLISM AND LEVELS OF INDOLE-3-ACETIC-ACID AND OTHER PHYTOHORMONES IN BEAN (PHASEOLUS-VULGARIS L) [J].

CAKMAK, I ;

MARSCHNER, H ;

BANGERTH, F .

JOURNAL OF EXPERIMENTAL BOTANY, 1989, 40 (212) :405-412

[3] Triticum dicoccoides:: An important genetic resource for increasing zinc and iron concentration in modern cultivated wheat [J].

Cakmak, I ;

Torun, A ;

Millet, E ;

Feldman, M ;

Fahima, T ;

Korol, A ;

Nevo, E ;

Braun, HJ ;

Özkan, H .

SOIL SCIENCE AND PLANT NUTRITION, 2004, 50 (07) :1047-1054

[4] Tansley review No. 111 - Possible roles of zinc in protecting plant cells from damage by reactive oxygen species [J].

Cakmak, I .

NEW PHYTOLOGIST, 2000, 146 (02) :185-205

[5] Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants [J].

Clemens, S. .

BIOCHIMIE, 2006, 88 (11) :1707-1719

[6] Put the metal to the petal: metal uptake and transport throughout plants [J].

Colangelo, Elizabeth P. ;

Guerinot, Mary Lou .

CURRENT OPINION IN PLANT BIOLOGY, 2006, 9 (03) :322-330

[7] Maize yellow stripe1 encodes a membrane protein directly involved in Fe(III) uptake [J].

Curie, C ;

Panaviene, Z ;

Loulergue, C ;

Dellaporta, SL ;

Briat, JF ;

Walker, EL .

NATURE, 2001, 409 (6818) :346-349

[8] The FRD3-mediated efflux of citrate into the root vasculature is necessary for efficient iron translocation [J].

Durrett, Timothy P. ;

Gassmann, Walter ;

Rogers, Elizabeth E. .

PLANT PHYSIOLOGY, 2007, 144 (01) :197-205

[9] PIC1, an ancient permease in Arabidopsis chloroplasts, mediates iron transport [J].

Duy, Daniela ;

Wanner, Gerhard ;

Meda, Anderson R. ;

von Wiren, Nicolaus ;

Soll, Juergen ;

Philippar, Katrin .

PLANT CELL, 2007, 19 (03) :986-1006

[10] A novel iron-regulated metal transporter from plants identified by functional expression in yeast [J].

Eide, D ;

Broderius, M ;

Fett, J ;

Guerinot, ML .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (11) :5624-5628

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