Characterization of monoclonal antibodies directed against the rat neurotensin receptor NTS1

被引：5

作者：

Niebauer, Ronald T. ^{[1
]}

White, Jim F. ^{[1
]}

Fei, Zhizhong ^{[1
]}

Grisshammer, Reinhard ^{[1
]}

机构：

[1] NIDDK, Mol Biol Lab, Dept Hlth & Human Serv, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION | 2006年 / 26卷 / 5-6期

关键词：

G protein-coupled receptor; Monoclonal Antibody; cocrystallization; neurotensin receptor; antibody receptor complex;

D O I：

10.1080/10799890600928228

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

G protein-coupled receptors (GPCRs) are integral membrane proteins that mediate cellular responses to a variety of ligands and represent major drug targets. Despite their medical importance, detailed structural information is limited because only one GPCR has been crystallized and its structure determined. To develop tools to aid in the formation of well-ordered crystals, we generated monoclonal antibodies with high affinity to the rat neurotensin receptor. All antibodies bound to the C-terminus of the receptor, which may reflect the selection strategy used to identify high-affinity binders. Further characterization revealed that some antibodies bound to the receptor in a sodium chloride sensitive manner, but others did not. Epitope mapping revealed distinct antigenic regions within the receptor C-terminus. Tight binding of Fab fragments to the receptor was verified by size exclusion chromatography.

引用

页码：395 / 415

页数：21

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