Expression and stability of two isoforms of ABCG1 in human vascular cells

被引：28

作者：

Gelissen, Ingrid C. ^{[2
]}

Cartland, Sian ^{[2
]}

Brown, Andrew J. ^{[3
]}

Sandoval, Cecilia ^{[2
]}

Kim, Mijurng ^{[2
]}

Dinnes, Donna Lee ^{[2
]}

Lee, Youra ^{[2
]}

Hsieh, Victar ^{[2
]}

Gaus, Katharina ^{[2
]}

Kritharides, Leonard ^{[2
,4
]}

Jessup, Wendy ^{[1
,2
]}

机构：

[1] Univ New S Wales, Sch Med Sci, Ctr Vasc Res, Sydney, NSW 2052, Australia

[2] Prince Wales Hosp, Dept Haematol, Sydney, NSW, Australia

[3] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW, Australia

[4] Concord Repatriat Gen Hosp, Dept Cardiol, Sydney, NSW, Australia

来源：

ATHEROSCLEROSIS | 2010年 / 208卷 / 01期

基金：

英国医学研究理事会;

关键词：

ABC transporter; Reverse cholesterol transport; Macrophage; Vascular endothelial cells; Smooth muscle cells; CASSETTE TRANSPORTER G1; HIGH-DENSITY-LIPOPROTEIN; DROSOPHILA-WHITE GENE; ATHEROSCLEROTIC LESION DEVELOPMENT; CELLULAR CHOLESTEROL EFFLUX; FATTY-ACIDS PHOSPHORYLATE; TYPE-2; DIABETES-MELLITUS; ENDOTHELIAL-CELLS; LIPID-ACCUMULATION; DESTABILIZE ABCA1;

D O I：

10.1016/j.atherosclerosis.2009.06.028

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

Objective: To evaluate the expression of two ABCG1 isoforms that differ in the presence or absence of a 12 amino acid (AA) peptide between the ABC cassette and the transmembrane region, termed ABCG1(+ 12) and ABCG1(-12), respectively, in human vascular cells and tissues. Methods and results: mRNA for both isoforms was expressed in human macrophages, vascular endothelial and smooth muscle cells as well as whole human spleen, lung, liver and brain tissue. However, ABCG1(+ 12) was not expressed in mouse tissues. 2D gel electrophoresis of ABCG1 protein indicated that both protein isoforms were expressed in human macrophages. Furthermore the half-lives of the two ABCG1 protein isoforms, stably expressed in CHOK1 cells, measured under basal conditions were different, suggesting the presence of a degradation or stabilising signal in or near the 12AA region of ABCG1(+ 12). Conclusion: ABCG1(+ 12) is an isoform of ABCG1 exclusively expressed in human cells at the RNA and protein level. As ABCG1(+ 12) is not expressed in mice, although mouse models are widely used to elucidate the function of ABCG1, further investigations into the importance of this human ABCG1 isoform are warranted. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：75 / 82

页数：8

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