Alginate-coated chitosan membrane for guided tissue regeneration

Chitosan membranes were first prepared by a thermally induced phase separation method and then alginate was coated on one side of the membranes by a modified dialysis apparatus to prepare alginate/chitosan membranes (A/C membranes). Electron spectroscopy for chemical analysis (ESCA), scanning electron microscope, and contact angle measurements were conducted to evaluate the surface characteristics. The mechanical strength, degradation behavior, and cell adhesion test were performed to evaluate the feasibility of using A/C membrane in guided tissue regeneration applications. The results revealed that alginates could effectively be coated onto the chitosan membrane. As observed in ESCA results, the N-atomic emission peak was decreased from originally 6.2% on the untreated chitosan surface to 2% on the alginate-treated surface. The contact angle decreased on the alginate-modified side substantially, compared with the untreated side (from 88.4 degrees to 34.2 degrees). The A/C membrane had a higher water content of 71.8% in comparison to the chitosan membrane of 61.8%. Consequently, A/C membrane became stiffer and had a higher Young's modulus and strength. After a 30-day in vitro shaking test, the weight of membranes was degraded to about 75% from the original. The 3T3 fibroblast cells showed less adhesion to alginate-modified side as compared to the untreated chitosan-side in cell adhesion test. (c) 2006 Wiley Periodicals, Inc.