Purification and properties of NADP-dependent shikimate dehydrogenase from Gluconobacter oxydans IFO 3244 and its application to enzymatic shikimate production

被引：19

作者：

Adachi, Osao ^{[1
]}

Ano, Yoshitaka ^{[1
]}

Toyama, Hirohide ^{[1
]}

Matsushita, Kazunobu ^{[1
]}

机构：

[1] Yamaguchi Univ, Fac Agr, Dept Biol Chem, Yamaguchi 7538515, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2006年 / 70卷 / 11期

关键词：

enzymatic shikimate production; Gluconobacter oxydans; osteltamivir; shikimate dehydrogenase;

D O I：

10.1271/bbb.60305

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NADP-Dependent shikimate dehydrogenae (SKDH, EC 1.1.1.25) was purified from. Gluconobacter oxydans IFO 3244. SKDH showed a single protein band on native-PAGE accompanying enzyme activity. It required NADP exclusively and catalyzed only the shuttle reaction between shikimate and 3-dehydroshikimate. The optimum pH for shikimate oxidation and 3-dehydroshikimate reduction was found at pH 10 and 7 respectively. SKDH proved to be a useful catalyst for shikimate production from 3-dehydroshikimate.

引用

页码：2786 / 2789

页数：4

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