Biochemical analysis of human breast tissues using Fourier-transform Raman spectroscopy

被引：61

作者：

Bitar, Renata Andrade ^{[1
]}

Martinho, Herculano da Silva ^{[1
]}

Tierra-Criollo, Carlos Julio ^{[1
]}

Zambelli Ramalho, Leandra Naira ^{[1
]}

Netto, Mario Mourao ^{[1
]}

Martin, Airton Abrahao ^{[1
]}

机构：

[1] UNIVAP, IP&D, Lab Biomed Vibrat Spect, BR-12244000 Sao Paulo, Brazil

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2006年 / 11卷 / 05期

基金：

巴西圣保罗研究基金会;

关键词：

Raman spectroscopy; breast cancer; optical biopsy;

D O I：

10.1117/1.2363362

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We employ Fourier-transform Raman spectroscopy to study normal and tumoral human breast tissues, including several subtypes of cancers. We analyzed 194 Raman spectra from breast tissues that were separated into 9 groups according to their corresponding histopathological diagnosis. The assignment of the relevant Raman bands enabled us to connect the several kinds of breast tissues (normal and pathological) to their corresponding biochemical moieties alterations and distinguish among 7 groups: normal breast, fibrocystic condition, duct carcinoma in situ, duct carcinoma in situ with necrosis, infiltrating duct carcinoma not otherwise specified, colloid infiltrating duct carcinoma, and invasive lobular carcinomas. We were able to establish the biochemical basis for each spectrum, relating the observed peaks to specific biomolecules that play a special role in the carcinogenesis process. This work is very useful for the premature optical diagnosis of a broad range of breast pathologies. We noticed that we were not able to differentiate inflammatory and medullary duct carcinomas from infiltrating duct carcinoma not otherwise specified. (c) 2006 Society of Photo-Optical Instrumentation Engineers.

引用

页数：8

共 32 条

[1]

[Anonymous], LEHNINGER PRINCIPLES

[2]

[Anonymous], 1991, Lasers in the Life Sciences

[3]

Breast cancer diagnosis using FT-RAMAN Spectroscopy [J].

Bitar, RA ;

Martin, AA ;

Criollo, CJT ;

Ramalho, LNZ .

Advanced Biomedical and Clinical Diagnostic Systems III, 2005, 5692 :147-154

[4]

NEOPLASTIC PROGRESSION IN ULCERATIVE-COLITIS - HISTOLOGY, DNA CONTENT, AND LOSS OF A P53 ALLELE [J].

BURMER, GC ;

RABINOVITCH, PS ;

HAGGITT, RC ;

CRISPIN, DA ;

BRENTNALL, TA ;

KOLLI, VR ;

STEVENS, AC ;

RUBIN, CE .

GASTROENTEROLOGY, 1992, 103 (05) :1602-1610

[5]

The use of Raman spectroscopy to identify and characterize transitional cell carcinoma in vitro [J].

Crow, P ;

Uff, JS ;

Farmer, JA ;

Wright, MP ;

Stone, N .

BJU INTERNATIONAL, 2004, 93 (09) :1232-1236

[6]

Ten-year risk of false positive screening mammograms and clinical breast examinations [J].

Elmore, G ;

Barton, MB ;

Moceri, VM ;

Polk, S ;

Arena, PJ ;

Fletcher, SW .

NEW ENGLAND JOURNAL OF MEDICINE, 1998, 338 (16) :1089-1096

[7]

RAMAN-SPECTROSCOPY OF NORMAL AND DISEASED HUMAN BREAST TISSUES [J].

FRANK, CJ ;

MCCREERY, RL ;

REDD, DCB .

ANALYTICAL CHEMISTRY, 1995, 67 (05) :777-783

[8]

The p53 pathway in breast cancer [J].

Gasco, M ;

Shami, S ;

Crook, T .

BREAST CANCER RESEARCH, 2002, 4 (02) :70-76

[9]

Melanoma diagnosis by Raman spectroscopy and neural networks: Structure alterations in proteins and lipids in intact cancer tissue [J].

Gniadecka, M ;

Philipsen, PA ;

Sigurdsson, S ;

Wessel, S ;

Nielsen, OF ;

Christensen, DH ;

Hercogova, J ;

Rossen, K ;

Thomsen, HK ;

Gniadecki, R ;

Hansen, LK ;

Wulf, HC .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2004, 122 (02) :443-449

[10]

Haka AS, 2002, CANCER RES, V62, P5375

← 1 2 3 4 →