O-GlcNAcylation enhances FOXO4 transcriptional regulation in response to stress

被引：26

作者：

Ho, Shiuh-Rong ^{[1
]}

Wang, Kai ^{[2
]}

Whisenhunt, Thomas R. ^{[1
]}

Huang, Ping ^{[3
]}

Zhu, Xiaolin ^{[1
]}

Kudlow, Jeffrey E. ^{[1
,2
,3
,4
]}

Paterson, Andrew J. ^{[1
,2
,4
]}

机构：

[1] Univ Alabama, Dept Cell Biol, Birmingham, AL 35294 USA

[2] Univ Alabama, Dept Med, Div Endocrinol Diabet & Metab, Birmingham, AL 35294 USA

[3] Univ Alabama, Dept Pharmacol & Toxicol, Birmingham, AL 35294 USA

[4] Univ Alabama, Dept Physiol & Biophys, Birmingham, AL 35294 USA

来源：

FEBS LETTERS | 2010年 / 584卷 / 01期

关键词：

OGT; NCOAT; Hydrogen peroxide; O-GlcNAc; p27Kip1; BETA-N-ACETYLGLUCOSAMINE; PROTEIN-KINASE-B; NUCLEOCYTOPLASMIC PROTEINS; P27(KIP1) EXPRESSION; FACTOR AFX; GLCNAC; CELLS; INHIBITION; ACTIVATION;

D O I：

10.1016/j.febslet.2009.11.059

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The FOXO4 transcription factor plays an important role in cell survival in response to oxidative stress. The regulation of FOXO4 is orchestrated by post-translational modi. cations including phosphorylation, acetylation, and ubiquitination. Here, we demonstrate that O-GlcNAcylation also contributes to the FOXO4-dependent oxidative stress response. We show that hydrogen peroxide treatment of HEK293 cells increases FOXO4 association with OGT, the enzyme that adds O-GlcNAc to proteins, causing FOXO4 O-GlcNAcylation and enhanced transcriptional activity under acute oxidative stress. O-GlcNAcylation is known to be protective for cells under stress conditions, including oxidative stress. Our data provide a mechanism of FOXO4 anti-oxidative protection through O-GlcNAcylation.

引用

页码：49 / 54

页数：6

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