Ghrelin stimulates angiogenesis in human microvascular endothelial cells: Implications beyond GH release

被引：60

作者：

Li, Aihua ^{[1
]}

Cheng, Guangli ^{[1
]}

Zhu, Geng Hui ^{[1
]}

Tarnawski, Andrzej S. ^{[1
]}

机构：

[1] Univ Calif Irvine, Dept Med, Div Gastroenterol, VA Long Beach Healthcare Syst, Long Beach, CA 90822 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 353卷 / 02期

关键词：

ghrelin; GHS-R-growth hormone secretagogue receptor; angiogenesis; endothelial cells; proliferation; migration; ERK2;

D O I：

10.1016/j.bbrc.2006.11.144

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ghrelin, a peptide hormone isolated from the stomach, releases growth hormone and stimulates appetite. Ghrelin is also expressed in pancreas, kidneys, cardiovascular system and in endothelial cells. The precise role of ghrelin in endothelial cell functions remains unknown. We examined the expression of ghrelin and its receptor (GHSR1) mRNAs and proteins in human microvascular endothelial cells (HMVEC) and determined whether ghrelin affects in these cells proliferation, migration and in vitro angiogenesis; and whether MAPK/ERK2 signaling is important for the latter action. We found that ghrelin and GHSR1 are constitutively expressed in HMVEC. Treatment of HMVEC with exogenous ghrelin significantly increased in these cells proliferation, migration, in vitro angiogenesis and ERK2 phosphorylation. MEK/ERK2 inhibitor, PD 98059 abolished ghrelin-induced in vitro angiogenesis. This is the first demonstration that ghrelin and its receptor are expressed in human microvascular endothelial cells and that ghrelin stimulates HMVEC proliferation, migration, and angiogenesis through activation of ERK2 signaling.

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收藏

页码：238 / 243

页数：6

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