Response heterogeneity of human macrophages to ATP is associated with P2X7 receptor expression but not to polymorphisms in the P2RX7 promoter

被引：19

作者：

Li, CM

Campbell, SJ

Kumararatne, DS

Hill, AVS

Lammas, DA ^{[1
]}

机构：

[1] Univ Birmingham, Sch Med, MRC Ctr Immune Regulat, Div Immun & Infect, Birmingham B15 2TT, W Midlands, England

[2] Univ Oxford, Wellcome Trust Ctr Human Genet, Oxford OX3 7BN, England

[3] Addenbrookes Hosp, Dept Clin Immunol, Cambridge CB2 2QQ, England

来源：

FEBS LETTERS | 2002年 / 531卷 / 02期

基金：

英国惠康基金;

关键词：

P2X(7); promoter; polymorphism; ATP; cell lysis; 1513;

D O I：

10.1016/S0014-5793(02)03424-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A region 2 kb upstream of exon 1 of the P2X(7) gene was sequenced using DNA from nine healthy individuals who exhibited three different ATP response phenotypes (i.e. high, low and interferon gamma-inducible). Five single nucleotide polymorphisms were identified within the nine donor promoter sequences but none were associated with a specific ATP response phenotype. A P2X7 loss of function polymorphism (1513 in exon 13) was also screened for within donor DNA but no response associations were identified. ATP response phenotype was positively associated with P2X7 receptor expression, as assessed by flow cytometry, but not with any identified receptor or promoter gene polymorphisms. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：127 / 131

页数：5

共 12 条

[1]

AKIYAMA Y, TFSEARCH SEARCHING T

[2] CELL-TYPE SPECIFIC ACTIVITY OF 2 GLUCOCORTICOID RESPONSIVE UNITS OF RAT TYROSINE AMINOTRANSFERASE GENE IS ASSOCIATED WITH MULTIPLE BINDING-SITES FOR C/EBP AND A NOVEL LIVER-SPECIFIC NUCLEAR FACTOR [J].