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Comparison of two whole genome amplification methods for STR genotyping of LCN and degraded DNA samples

被引：49
作者：
Ballantyne, Kaye N.
van Oorschot, Roland A. H.
Mitchell, R. John
机构：
[1] Victoria Police Forens Serv Dept, Macleod, Vic 3085, Australia
[2] La Trobe Univ, Dept Genet, Bundoora, Vic 3086, Australia
来源：
FORENSIC SCIENCE INTERNATIONAL | 2007年 / 166卷 / 01期
基金：
澳大利亚研究理事会;
关键词：
whole genome amplification; LCN DNA; degraded DNA; multiple displacement amplification;
D O I：
10.1016/j.forsciint.2006.03.022
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
The analysis of LCN or highly degraded DNA samples presents a challenge for forensic science. Improving the quantity and/or quality of samples would greatly increase the profiling success rate from LCN and degraded samples. Whole genome amplification (WGA) is one method that has such potential. Two commercially available WGA kits, GenomePlex and GenomiPhi, were investigated for use on LCN and degraded DNA samples. Both kits amplified genomic DNA, producing microgram quantities from sub-nanogram templates. Profiling success of LCN DNA samples was increased, with improvements of over 700% from 10 pg template DNA compared to non-WGA-amplified control samples. The amplification success with degraded DNA was also improved by WGA. Degraded DNA was simulated using restriction enzymes to demonstrate that the application of WGA can result in the typing of STR loci that could not previously be amplified. An increase in artefacts, such as stutter alleles and amplification biases, were observed in many samples. Results show that WGA is capable of increasing both the quality and quantity of DNA, and has the potential to improve profiling success from difficult samples in forensic casework. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
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页码：35 / 41
页数：7
相关论文
共 16 条
[1]   Comprehensive human genome amplification using multiple displacement amplification [J].
Dean, FB ;
Hosono, S ;
Fang, LH ;
Wu, XH ;
Faruqi, AF ;
Bray-Ward, P ;
Sun, ZY ;
Zong, QL ;
Du, YF ;
Du, J ;
Driscoll, M ;
Song, WM ;
Kingsmore, SF ;
Egholm, M ;
Lasken, RS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (08) :5261-5266
[2]   An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA [J].
Gill, P ;
Whitaker, J ;
Flaxman, C ;
Brown, N ;
Buckleton, J .
FORENSIC SCIENCE INTERNATIONAL, 2000, 112 (01) :17-40
[3]  
Green RL, 2005, J FORENSIC SCI, V50, P809
[4]   Isothermal whole genome amplification from single and small numbers of cells: a new era for preimplantation genetic diagnosis of inherited disease [J].
Handyside, AH ;
Robinson, MD ;
Simpson, RJ ;
Omar, MB ;
Shaw, MA ;
Grudzinskas, JG ;
Rutherford, A .
MOLECULAR HUMAN REPRODUCTION, 2004, 10 (10) :767-772
[5]   Whole genome amplification strategy for forensic genetic analysis using single or few cell equivalents of genomic DNA [J].
Hanson, EK ;
Ballantyne, J .
ANALYTICAL BIOCHEMISTRY, 2005, 346 (02) :246-257
[6]   Multiple displacement amplification on single cell and possible PGD applications [J].
Hellani, A ;
Coskun, S ;
Benkhalifa, M ;
Tbakhi, A ;
Sakati, N ;
Al-Odaib, A ;
Ozand, P .
MOLECULAR HUMAN REPRODUCTION, 2004, 10 (11) :847-852
[7]   Unbiased whole-genome amplification directly from clinical samples [J].
Hosono, S ;
Faruqi, AF ;
Dean, FB ;
Du, YF ;
Sun, ZY ;
Wu, XH ;
Du, J ;
Kingsmore, SF ;
Egholm, M ;
Lasken, RS .
GENOME RESEARCH, 2003, 13 (05) :954-964
[8]   Genome amplification of single sperm using multiple displacement amplification [J].
Jiang, ZW ;
Zhang, XQ ;
Deka, R ;
Jin, L .
NUCLEIC ACIDS RESEARCH, 2005, 33 (10) :1-9
[9]   Quantitative evaluation by minisequencing and microarrays reveals accurate multiplexed SNP genotyping of whole genome amplified DNA -: art. no. e129 [J].
Lovmar, L ;
Fredriksson, M ;
Liljedahl, U ;
Sigurdsson, S ;
Syvänen, AC .
NUCLEIC ACIDS RESEARCH, 2003, 31 (21) :e129
[10]  
MURTHY KK, 2005, HUM MUTAT, V26, P1
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