Apical gene transfer into quiescent human and canine polarized intestinal epithelial cells by lentivirus vectors

被引：34

作者：

Seppen, J

Barry, SC

Klinkspoor, JH

Katen, LJ

Lee, SP

Garcia, JV

Osborne, WRA

机构：

[1] Univ Washington, Sch Med, Dept Pediat, Seattle, WA 98195 USA

[2] Univ Washington, Dept Gastroenterol, Seattle, WA 98195 USA

[3] Vet Affairs Med Ctr, Seattle, WA 98108 USA

[4] Univ Texas, SW Med Ctr, Dept Internal Med, Dallas, TX USA

来源：

JOURNAL OF VIROLOGY | 2000年 / 74卷 / 16期

关键词：

D O I：

10.1128/JVI.74.16.7642-7645.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Intestinal epithelial cells secrete a protective luminal mucus barrier inhibiting viral gene transfer. Quiescent, polarized monolayers of primary epithelial cells from dog gallbladder and human colon are efficiently transduced through the apical mucus side by lentivirus vectors, suggesting their application to intestinal gene therapy.

引用

收藏

页码：7642 / 7645

页数：4

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