Parallel-up structure evidences the molecular directionality during biosynthesis of bacterial cellulose

被引:211
作者
Koyama, M
Helbert, W
Imai, T
Sugiyama, J
Henrissat, B
机构
[1] KYOTO UNIV,WOOD RES INST,UJI,KYOTO 611,JAPAN
[2] UNIV GRENOBLE 1,CNRS,CTR RECH MACROMOL VEGETALES,F-38041 GRENOBLE 9,FRANCE
关键词
reducing-end staining; microdiffraction; cellulose I-alpha; cellulose I-beta; chain polarity;
D O I
10.1073/pnas.94.17.9091
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The ''parallel-up'' packing in cellulose I-alpha and I-beta unit cells was experimentally demonstrated by a combination of direct-staining the reducing ends of cellulose chains and microdiffraction-tilting electron crystallographic analysis. Microdiffraction investigation of nascent bacterial cellulose microfibrils showed that the reducing end of the growing cellulose chains points away from the bacterium, and this provides direct evidence that polymerization by the cellulose synthase takes place at the nonreducing end of the growing cellulose chains, This mechanism is likely to be valid also for a number of processive glycosyltransferases such as chitin synthases, hyaluronan synthases, and proteins involved in the synthesis of nodulation factor backbones.
引用
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页码:9091 / 9095
页数:5
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