Recombinant env-gp85 of HPRS-1OS (subgroup J) avian leukosis virus: Antigenic characteristics and usefulness as a diagnostic reagent

被引：38

作者：

Venugopal, K

Howes, K

Barron, GS

Payne, LN

机构：

[1] Institute for Animal Health, Nr. Newbury

来源：

AVIAN DISEASES | 1997年 / 41卷 / 02期

关键词：

avian leukosis virus; gp85; baculovirus; glutathione-S-transferase;

D O I：

10.2307/1592179

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We describe the construction of a recombinant baculovirus containing the cloned DNA encoding the gp85 envelope glycoprotein of HPRS-103 (subgroup J) avian leukosis virus fused to the carboxy-terminus of the affinity tag glutathione-S-transferase. The fusion protein was efficiently secreted into the supernatant medium of the infected insect cell culture and could be purified in a single step using immobilized glutathione. An enzyme-linked immunosorbent assay using the recombinant protein was found to be specific and sensitive for detection of HPRS-103 virus-specific antibodies in the sera of infected birds.

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页码：283 / 288

页数：6

相关论文

共 14 条

[1] HPRS-103 (EXOGENOUS AVIAN-LEUKOSIS VIRUS, SUBGROUP-J) HAS AN ENV GENE-RELATED TO THOSE OF ENDOGENOUS ELEMENTS EAV-0 AND E51 AND AN E-ELEMENT FOUND PREVIOUSLY ONLY IN SARCOMA-VIRUSES [J].

BAI, J ;

PAYNE, LN ;

SKINNER, MA .

JOURNAL OF VIROLOGY, 1995, 69 (02) :779-784

[2] MULTIPLE COMPLEX FAMILIES OF ENDOGENOUS RETROVIRUSES ARE HIGHLY CONSERVED IN THE GENUS GALLUS [J].

BOYCEJACINO, MT ;

ODONOGHUE, K ;

FARAS, AJ .

JOURNAL OF VIROLOGY, 1992, 66 (08) :4919-4929

[3] LINEARIZATION OF BACULOVIRUS DNA ENHANCES THE RECOVERY OF RECOMBINANT VIRUS EXPRESSION VECTORS [J].

KITTS, PA ;

AYRES, MD ;

POSSEE, RD .

NUCLEIC ACIDS RESEARCH, 1990, 18 (19) :5667-5672

[4] EXPRESSION OF GLYCOPROTEIN-D OF HERPES-SIMPLEX VIRUS TYPE-1 IN A RECOMBINANT BACULOVIRUS - PROTECTIVE RESPONSES AND T-CELL RECOGNITION OF THE RECOMBINANT-INFECTED CELL-EXTRACTS [J].

KRISHNA, S ;

BLACKLAWS, BA ;

OVERTON, HA ;

BISHOP, DHL ;

NASH, AA .

JOURNAL OF GENERAL VIROLOGY, 1989, 70 :1805-1814

[5] THE OLIGOSACCHARIDES OF INFLUENZA-VIRUS HEMAGGLUTININ EXPRESSED IN INSECT CELLS BY A BACULOVIRUS VECTOR [J].

KURODA, K ;

GEYER, H ;

GEYER, R ;

DOERFLER, W ;

KLENK, HD .

VIROLOGY, 1990, 174 (02) :418-429

[6] BACULOVIRUS EXPRESSION VECTORS - THE REQUIREMENTS FOR HIGH-LEVEL EXPRESSION OF PROTEINS, INCLUDING GLYCOPROTEINS [J].

MATSUURA, Y ;

POSSEE, RD ;

OVERTON, HA ;

BISHOP, DHL .

JOURNAL OF GENERAL VIROLOGY, 1987, 68 :1233-1250

[7]

MIZUNO Y, 1986, AM J VET RES, V47, P551

[8] EXPRESSION OF AVIAN LEUKEMIA-VIRUS ENV-GP85 IN SPODOPTERA-FRUGIPERDA CELLS BY USE OF A BACULOVIRUS EXPRESSION VECTOR [J].

NOTEBORN, MHM ;

DEBOER, GF ;

KANT, A ;

KOCH, G ;

BOS, JL ;

ZANTEMA, A ;

VANDEREB, AJ .

JOURNAL OF GENERAL VIROLOGY, 1990, 71 :2641-2648

[9] A NOVEL SUBGROUP OF EXOGENOUS AVIAN-LEUKOSIS VIRUS IN CHICKENS [J].

PAYNE, LN ;

BROWN, SR ;

BUMSTEAD, N ;

HOWES, K ;

FRAZIER, JA ;

THOULESS, ME .

JOURNAL OF GENERAL VIROLOGY, 1991, 72 :801-807

[10] HOST RANGE OF ROUS-SARCOMA VIRUS PSEUDOTYPE RSV(HPRS-103) IN 12 AVIAN SPECIES - SUPPORT FOR A NEW AVIAN RETROVIRUS ENVELOPE SUBGROUP, DESIGNATED-J [J].

PAYNE, LN ;

HOWES, K ;

GILLESPIE, AM ;

SMITH, LM .

JOURNAL OF GENERAL VIROLOGY, 1992, 73 :2995-2997