The stimulatory effect of opioids on cyclic amp production in SK-N-SH cells is mediated by calcium ions

被引：19

作者：

Fields, A ^{[1
]}

Same, Y ^{[1
]}

机构：

[1] TEL AVIV UNIV, SACKLER SCH MED, DEPT PHYSIOL & PHARMACOL, MAUERBERGER CHAIR NEUROPHARMACOL, IL-69978 TEL AVIV, ISRAEL

来源：

LIFE SCIENCES | 1997年 / 61卷 / 06期

关键词：

opioids; cyclic AMP; calcium; calmodulin; neuroblastoma;

D O I：

10.1016/S0024-3205(97)00434-7

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The present study examines the stimulatory effect of opioids on adenosine 3':5'-cyclic monophosphate (cyclic AMP) production in the human neuroblastoma cell line SK-N-SH, and its dependence on calcium. We show that, in this culture, the mu-opioid selective agonist [D-Ala(2), N-Me-Phe(4), Gly(5)-ol]-Enkephalin stimulates cyclic AMP production by 30% in a naloxone-reversible manner. This stimulation is completely dependent on calcium and involves the activation of calcium/calmodulin since it is abolished in the presence of EGTA, calcium channel blockers or N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride (W-7). The results suggest that the activation of calcium/calmodulin dependent adenylyl cyclases by opioids in SK-N-SH cells is secondary to the induction of calcium influx and the consequent elevation of intracellular calcium level.

引用

页码：595 / 602

页数：8

共 39 条

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