Time-shaved X(ω1)-half-filter for improved sensitivity in subspectral editing

Experiments with X-half-filter elements allow the separation of the resonances from protons bound and unbound to a spin X into different subspectra, This Communication presents a modified half-filter element where the filter delay is simultaneously used for chemical shift labeling and scalar coupling evolution in a semi-constant time experiment, The filter element is demonstrated with a H-1 NOESY spectrum of a 28.5-kDa 2:1 complex between the uniformly C-13-labeled N-terminal domain of Escherichia coli arginine repressor and operator DNA, (C) 2000 Academic Press.